Auxin Starvation Results in G2-Arrest in Suspension-Cultured Tobacco Cells

• Published in: Journal of plant physiology Vol. 147; no. 3; pp. 391 - 396
• Main Authors: Koens, K.B., Nicoloso, F.T., Harteveld, M., Libbenga, K.R., Kijne, J.W.
• Format: Journal Article
• Language: English
• Published: Jena Elsevier GmbH 1995; Elsevier
• Subjects: 2,4-D; 2,4-Dichlorphenoxiessigsaeure; 6-BENZILADENINA; 6-BENZYLADENINE; AUXINAS; AUXINE; AUXINS; Benzylaminopurin; Biological and medical sciences; CELL CULTURE; cell cycle; Cell kinetics; Cell physiology; cell suspension cultures; CELLS; CELLULE; CELULAS; CITOQUININAS; CULTIVO DE CELULAS; CULTURE DE CELLULE; CYTOKININE; CYTOKININS; Fundamental and applied biological sciences. Psychology; HORMONAS; HORMONE; HORMONES; MITOSE; MITOSIS; NICOTIANA TABACUM; Nicotiana tabacum L; Pflanzenhormon; PLANT GROWTH SUBSTANCES; Plant physiology and development; Proliferation; SUBSTANCE DE CROISSANCE VEGETALE; SUSTANCIAS DE CRECIMIENTO VEGETAL; Wuchsstoff; Zellsprossung; Zellsuspensionskultur; Zellzyklus; 3,5-D; BAP; FDA; TIBA; 2,4-D; cell cycle; DMSO; cell suspension cultures; LS; Nicotiana tabacum L; NAA; Cell culture; Flow cytometry; Auxin; Mitosis; Nicotiana tabacum; Cytokinin; Dicotyledones; Angiospermae; Cell cycle; Spermatophyta; Phytohormone; Solanaceae
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/S0176-1617(11)82173-5

In cell suspensions of tobacco LT cells, cultured on Linsmaier and Skoog medium with 2,4-dichlorophenoxyacetic acid (2,4-D) as the sole plant growth regulator, final cell number is dependent on the initial concentration of 2,4-D in the medium. At low concentrations of 2,4-D, accumulation of cells in the G2-phase of the cell cycle appeared to increase. Cells arrested in the G2-phase could enter mitosis and continue cycling after addition of 2,4-D or the auxin analogue NAA. Addition of cytokinins or 3,5-D did not enable paßage of the cells into mitosis. This suggests that auxin (analogues) influence a restriction point in the G2-phase of the cell cycle in tobacco LT cells.