Liquid Biopsy as a Diagnostic and Prognostic Tool for Women and Female Dogs with Breast Cancer

• Published in: Cancers Vol. 13; no. 20; p. 5233
• Main Authors: Colombo, Jucimara, Moschetta-Pinheiro, Marina Gobbe, Novais, Adriana Alonso, Stoppe, Bruna Ribeiro, Bonini, Enrico Dumbra, Gonçalves, Francine Moraes, Fukumasu, Heidge, Coutinho, Luiz Lehmann, Chuffa, Luiz Gustavo de Almeida, Zuccari, Debora Aparecida Pires de Campos
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 19-10-2021; MDPI
• Subjects: AKT1 protein; Biopsy; BRCA1 protein; BRCA2 protein; Breast cancer; Cancer therapies; Clinical medicine; Cloning; Diagnosis; Dogs; ErbB-2 protein; Ethics; Genetic diversity; Genetic testing; Genomes; Insulin-like growth factor II receptors; Metastases; Metastasis; MLH1 protein; Mortality; Mutation; Next-generation sequencing; Patients; Plasma; Tumors; USH2A protein; Womens health; United States > US; next generation sequencing (NGS); breast cancer; circulating tumor DNA (ctDNA); liquid biopsy; gene variant
• ISSN: 2072-6694; 2072-6694
• DOI: 10.3390/cancers13205233

Breast cancer (BC) is the malignant neoplasm with the highest mortality rate in women and female dogs are good models to study BC.
We investigated the efficacy of liquid biopsy to detect gene mutations in the diagnosis and follow-up of women and female dogs with BC.
In this study, 57 and 37 BC samples were collected from women and female dogs, respectively. After core biopsy and plasma samples were collected, the DNA and ctDNA of the tumor fragments and plasma were processed for next generation sequencing (NGS) assay. After preprocessing of the data, they were submitted to the Genome Analysis ToolKit (GATK).
In women, 1788 variants were identified in tumor fragments and 221 variants in plasma; 66 variants were simultaneously detected in tumors and plasma. Conversely, in female dogs, 1430 variants were found in plasma and 695 variants in tumor fragments; 59 variants were simultaneously identified in tumors and plasma. The most frequently mutated genes in the tumor fragments of women were
,
, and
; in female dogs, they were
,
, and
. Plasma of women showed the most frequent genetic variations in the MAP3K1, BRCA1, and GRB7 genes, whereas plasma from female dogs had variations in the
,
, and
genes. Mutations in the
,
, and
genes were associated with tumor recurrence, with a highly pathogenic variant in
being particularly prominent. We also detected a gain-of-function mutation in the
,
, and
genes.
Liquid biopsy is useful to identify specific genetic variations at the beginning of BC manifestation and may be accompanied over the entire follow-up period, thereby supporting the clinicians in refining interventions.