Nitric oxide regulates body temperature, neuronal activation and interleukin-1β gene expression in the hypothalamic paraventricular nucleus in response to immune stress

• Published in: Neuropharmacology Vol. 39; no. 11; pp. 2075 - 2089
• Main Authors: Yang, Wendy W, Krukoff, Teresa L
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01-10-2000; Elsevier
• Subjects: Analysis of the immune response. Humoral and cellular immunity; Animals; Biological and medical sciences; Blood Pressure - drug effects; Blood Pressure - physiology; Body Temperature - drug effects; Body Temperature - physiology; c- fos; Endothelial nitric oxide synthase; Endotoxins; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Gene Expression - drug effects; Gene Expression - physiology; Immunobiology; interleukin 1^b; Interleukin-1 - metabolism; Lipopolysaccharide; Lipopolysaccharides; Male; Miscellaneous; NADP - metabolism; NADPH-d; Neurons - drug effects; Neurons - physiology; Nitric Oxide - physiology; Nitric Oxide Synthase - antagonists & inhibitors; Nitric Oxide Synthase - drug effects; Nitric Oxide Synthase - metabolism; Nitric Oxide Synthase Type III; Paraventricular Hypothalamic Nucleus - drug effects; Paraventricular Hypothalamic Nucleus - physiology; Rat; Rats; Rats, Sprague-Dawley; Regulatory factors and their cellular receptors; Stress, Physiological - chemically induced; Stress, Physiological - physiopathology; Lipopolysaccharide; Endothelial nitric oxide synthase; Rat; NADPH-d; c- fos; Immune response; Enzyme; Body temperature; Cytokine; Central nervous system; Interleukin 1β; Action potential; Hypothalamus; Gene expression; Biological activity; Nitric-oxide synthase; Nitric oxide; Intracerebral administration; Oxidoreductases; Mechanism of action; Brain (vertebrata); Immune system
• ISSN: 0028-3908; 1873-7064
• DOI: 10.1016/S0028-3908(00)00054-X

An immune challenge initiates a complex cascade of events in the body including important responses from the central nervous system. As nitric oxide (NO) has been implicated in the central regulation of neuroendocrine and autonomic responses, this study was performed to determine if NO regulates physiological responses, neuronal activation, and/or interleukin-1β (IL-1β) gene expression in the paraventricular nucleus of the rat hypothalamus (PVN) in response to intravenous endotoxin, lipopolysaccharide (LPS, 100 μg/kg). Intracerebroventricular injections of NO synthase (NOS) inhibitors (7-nitroindazole sodium salt for neuronal NOS, N
G-nitro-L-arginine for neuronal NOS and endothelial NOS, and aminoguanidine for inducible NOS) in LPS-treated rats showed that inhibition of NOS eliminated the drop in body temperature and led to increased neuronal activation in the PVN as assessed by immunohistochemistry for Fos-like immunoreactivity. Activation of NO-producing PVN neurons was also increased in these rats suggesting that NO influences neuronal NOS activity in PVN neurons. Finally, increased IL-1β gene expression in the PVN of LPS-treated rats receiving N
G-nitro-L-arginine showed that NO regulates brain IL-1β gene expression. The results obtained with the NOS inhibitors support the hypothesis that NO produced from eNOS in the brain participates in temperature regulation, and inhibits PVN neuronal activity and IL-1β gene expression during immune stress.