Transtentorial cerebellar c- jun expression after focal cerebral cortical injury in mice

• Published in: Neuroscience letters Vol. 282; no. 1; pp. 85 - 88
• Main Authors: Liu, Jia-Shou, Chang, Yung-Yee, Wu, Hsiu-Shan, Huang, Chiung-Ying, Chen, Wei-Hsi, Lan, Min-Yu, Kao, Yi-Fen, Chen, Shun-Sheng
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 17-03-2000; Elsevier
• Subjects: Animals; Biochemistry and metabolism; Biological and medical sciences; Brain Injuries - etiology; Brain Injuries - metabolism; Brain Injuries - pathology; c- jun; Central nervous system; Cerebellum; Cerebellum - metabolism; Cold Temperature - adverse effects; Cryogenic cortical injury; Fundamental and applied biological sciences. Psychology; Immunohistochemistry; In Situ Hybridization; Male; Mice; Parietal Lobe - pathology; Proto-Oncogene Proteins c-jun - metabolism; Purkinje Cells - metabolism; Remote effect; RNA, Messenger - metabolism; Time Factors; Vertebrates: nervous system and sense organs; Cerebellum; Immunohistochemistry; Cryogenic cortical injury; In situ hybridization; Remote effect; c- jun; Vertebrata; Cerebral cortex; Mammalia; Mouse; Rodentia; Central nervous system; Lesion; Gene expression; Protooncogene; Brain (vertebrata); Immediate early gene
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/S0304-3940(00)00865-X

Delayed and remote effect of focal cerebral cortical lesion on cerebellum remains unclear. The c-Jun, an inducible transcription factor of cellular immediate early gene, is the predominant transcription factor and consistent marker for neurons that respond to stress or injury. We use a mouse cryogenic injury model to study the spatial and temporal changes of c-
jun in the cerebellum after focal neocortical lesion. A transient and moderate expression of c-
jun mRNA was found in the cerebellum with central dominance since 3 day postinjury and gradually subsided within 2 weeks. A distinct increment of c-Jun protein expression in Purkinje cells of the bilateral cerebellar hemispheres with focal connotation in the vermis was detected since 1 week postinjury. These findings suggest that the delayed and remote c-
jun expression of the cerebellum, functionally connected with the cerebral cortex, indicate transneuronal gene activation.