Effects of carbon and nitrogen sources on the proteome of Pseudomonas aeruginosa PA1 during rhamnolipid production

• Published in: Process biochemistry (1991) Vol. 45; no. 9; pp. 1504 - 1510
• Main Authors: Reis, Rodrigo Siqueira, da Rocha, Surza Lucia Gonçalves, Chapeaurouge, Donat Alexander, Domont, Gilberto Barbosa, Santa Anna, Lidia Maria Melo, Freire, Denise Maria Guimarães, Perales, Jonas
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-09-2010
• Subjects: Bioremediation; Carbon; Carbon and nitrogen source; Cellular; Control; Genetics; Proteins; Proteomics; Pseudomonas aeruginosa; Rhamnolipid; Rhamnolipids; Secretions; Pseudomonas aeruginosa; Rhamnolipid; Bioremediation; Carbon and nitrogen source; Proteomics
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2010.05.032

The PA1 strain of
Pseudomonas aeruginosa isolated from oil waste produces rhamnolipid, a biodegradable surfactant with applications in several industrial and environmental fields. The metabolic pathway and genetic regulation of rhamnolipid production in
P. aeruginosa are poorly understood. Herein, several proteins directly or indirectly related to rhamnolipid production and their genetic regulations were identified by comparative proteomic. We compared the proteome of
P. aeruginosa PA1 after fermentation in two different conditions of carbon and nitrogen sources: condition A allowed rhamnolipid production and condition B prevented it. Protein extracts from cellular pellets were compared using 2D-PAGE stained with colloidal Coomassie followed by MALDI-TOF/TOF mass spectrometry. We identified 21 differentially expressed proteins, including those involved in secretion,
quorum sensing, oxidative response and metabolism.