Effects of octreotide on acute necrotizing pancreatitis in rabbits

• Published in: World journal of gastroenterology : WJG Vol. 10; no. 14; pp. 2082 - 2086
• Main Authors: Czakó, László, Hegyi, Péter, Takács, Tamás, Góg, Csaba, Farkas, András, Mándy, Yvette, Varga, Ilona Sz, Tiszlavicz, László, Lonovics, János
• Format: Journal Article
• Language: English
• Published: United States First Department of Internal Medicine,University of Szeged,Szeged,Hungary%Second Department of Internal Medicine and Cardiological Center,University of Szeged,Szeged,Hungary%Department of Microbiology,University of Szeged,Szeged,Hungary%Biological Isotope Laboratory,Attila József University,Szeged,Hungary%Department of Pathology,University of Szeged,Szeged,Hungary 15-07-2004; Baishideng Publishing Group Inc
• Subjects: Animals; Basic Research; Cytokines - antagonists & inhibitors; Male; Octreotide - pharmacology; Pancreas - drug effects; Pancreas - metabolism; Pancreas - pathology; Pancreatitis, Acute Necrotizing - chemically induced; Pancreatitis, Acute Necrotizing - pathology; Pancreatitis, Acute Necrotizing - prevention & control; Rabbits; Reactive Oxygen Species - antagonists & inhibitors; Taurocholic Acid
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v10.i14.2082

To assess the role of oxygen-derived free radicals and cytokines in the pathogenesis of taurocholic acid-induced acute pancreatitis, and to evaluate the preventive effects of octreotide towards the development of acute pancreatitis.
Acute pancreatitis was induced in male New Zealand white rabbits by retrograde injection of 0.8 mL/kg.b.m. of 50 g/L sodium taurocholate (NaTC) in the pancreatic duct. Sham-operated animals served as control. Octreotide 1 mg/kg.b.m. was administered subcutaneously before the induction of pancreatitis. Blood was taken from the jugular vein before and at 1, 3, 6, 12 and 24 h after pancreatitis induction. Serum activities of amylase, IL-6 and TNF-alpha and levels of malonyl dialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GPx), catalase and superoxide dismutase (Mn-, Cu-, and Zn-SOD) in pancreatic tissue were measured.
Serum TNF-alpha and IL-6 levels increased significantly 3 h after the onset of pancreatitis, and then returned to control level. The tissue concentration of MDA was significantly elevated at 24 h, while the GSH level and GP-x, catalase, Mn-SOD, Cu-, Zn-SOD activities were all significantly decreased in animals with pancreatitis as compared to the control. Octreotide pretreatment significantly reversed the changes in cytokines and reactive oxygen metabolites. Octreotide treatment did not alter the serum amylase activity and did not have any beneficial effects on the development of histopathological changes.
Oxygen-derived free radicals and proinflammatory cytokines are generated at an early stage of NaTc-induced acute pancreatitis in rabbits. Prophylactic octreotide treatment can prevent release of cytokines and generation of reactive oxygen metabolites, but does not have any beneficial effects on the development of necrotizing pancreatitis.