Determination of fluoroquinolone antibiotics by microchip capillary electrophoresis along with time-resolved sensitized luminescence of their terbium(III) complexes

• Published in: Mikrochimica acta (1966) Vol. 181; no. 15-16; pp. 1897 - 1904
• Main Authors: Sierra-Rodero, Marina, Fernández-Romero, Juan Manuel, Gómez-Hens, Agustina
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01-11-2014; Springer
• Subjects: Analysis; Analytical Chemistry; Antibacterial agents; Antibiotics; Capillarity; Characterization and Evaluation of Materials; Chelates; Chelation; Chemistry; Chemistry and Materials Science; Electrophoresis; Ethylenediaminetetraacetic acid; Extraction; Integrated circuits; Luminescence; Microengineering; Microfluidics; Nanochemistry; Nanotechnology; Original Paper; Semiconductor chips; Solid phases; Fluoroquinolones; Laser induced luminescence; Microchip electrophoresis; Time-resolved-terbium sensitized luminescence; Water samples
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-014-1266-x

We report on the time-resolved detection of the three fluoroquinolone (FQs) antibiotics ciprofloxacin (CIP), enrofloxacin (ENR) and flumequine (FLU). On addition of terbium(III) ions, the terbium(III)-FQs chelates are formed in-situ in an on-capillary derivatization reaction of a microfluidic system. The laser-induced terbium(III)-sensitized luminescence of the chelates is measured at excitation/emission wavelengths of 337/545 nm. The analytes can be separated and quantified within less than 4 min. A solid phase extraction step for analyte preconcentration can be included prior to chelation and microchip capillary electrophoresis. The analytical ranges of the calibration graphs for CIP, ENR and FLU are from 10.6 to 60.0, 10.3 to 51.0, and 11.5 to 58.8 ng mL
−1
, respectively, and the detection limits are 3.2, 3.1 and 3.6 ng mL
−1
, respectively. The precision was established at two concentration levels of each analyte and revealed relative standard deviations in the range from 3.0 to 10.2 %. The method was applied to the analysis of FQ-spiked water samples.
Figure
We report on the time-resolved detection of the three fluoroquinolone antibiotics. The analytes can be separated and quantified within less than 4 min. A solid phase extraction step for analyte preconcentration can be included prior to chelation and microchip capillary electrophoresis.