Bulk segregant analysis of an induced floral mutant identifies a MIXTA-like R2R3 MYB controlling nectar guide formation in Mimulus lewisii

The genetic and developmental basis of many ecologically important floral traits (e.g., carotenoid pigmentation, corolla tube structure, nectar volume, pistil and stamen length) remains poorly understood. Here we analyze a chemically induced floral mutant of Mimulus lewisii through bulk segregant an...

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Bibliographic Details
Published in:	Genetics (Austin) Vol. 194; no. 2; pp. 523 - 528
Main Authors:	Yuan, Yao-Wu, Sagawa, Janelle M, Di Stilio, Verónica S, Bradshaw, Jr, H D
Format:	Journal Article
Language:	English
Published:	United States Genetics Society of America 01-06-2013
Subjects:	Crosses, Genetic Developmental biology Flowers & plants Flowers - anatomy & histology Flowers - genetics Genetic diversity Mimulus - genetics Mimulus lewisii Mutation Pigmentation - genetics Plant biology Plant Proteins - genetics Studies Transcription Factors - genetics trichome nectar guides carotenoids MIXTA-like MYB Mimulus
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Summary:	The genetic and developmental basis of many ecologically important floral traits (e.g., carotenoid pigmentation, corolla tube structure, nectar volume, pistil and stamen length) remains poorly understood. Here we analyze a chemically induced floral mutant of Mimulus lewisii through bulk segregant analysis and transgenic experiments and identify a MIXTA-like R2R3 MYB gene that controls nectar guide formation in M. lewisii flowers, which involves epidermal cell development and carotenoid pigmentation.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 Supporting information is available online at http://www.genetics.org/lookup/suppl/doi:10.1534/genetics.113.151225/-/DC1. Short read data have been deposited in the NCBI Short Read Archive (SRA056512); annotated GUIDELESS and other MIXTA-like gene sequences have been deposited in GenBank (KC139356 and KC692454–KC692460).
ISSN:	1943-2631 0016-6731 1943-2631
DOI:	10.1534/genetics.113.151225