An autonomously swimming biohybrid fish designed with human cardiac biophysics

Biohybrid systems have been developed to better understand the design principles and coordination mechanisms of biological systems. We consider whether two functional regulatory features of the heart-mechanoelectrical signaling and automaticity-could be transferred to a synthetic analog of another f...

Full description

Saved in:

Bibliographic Details
Published in:	Science (American Association for the Advancement of Science) Vol. 375; no. 6581; pp. 639 - 647
Main Authors:	Lee, Keel Yong, Park, Sung-Jin, Matthews, David G, Kim, Sean L, Marquez, Carlos Antonio, Zimmerman, John F, Ardoña, Herdeline Ann M, Kleber, Andre G, Lauder, George V, Parker, Kevin Kit
Format:	Journal Article
Language:	English
Published:	United States The American Association for the Advancement of Science 11-02-2022
Subjects:	Actuation Animal Fins - physiology Animals Automation Biomechanical Phenomena Biomimetics Biophysics Fish Fishes - physiology Humans Muscle Contraction Muscles Muscles - physiology Myocytes, Cardiac - physiology Rhythms Robotics Signaling Swimming Tissue Engineering
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Biohybrid systems have been developed to better understand the design principles and coordination mechanisms of biological systems. We consider whether two functional regulatory features of the heart-mechanoelectrical signaling and automaticity-could be transferred to a synthetic analog of another fluid transport system: a swimming fish. By leveraging cardiac mechanoelectrical signaling, we recreated reciprocal contraction and relaxation in a muscular bilayer construct where each contraction occurs automatically as a response to the stretching of an antagonistic muscle pair. Further, to entrain this closed-loop actuation cycle, we engineered an electrically autonomous pacing node, which enhanced spontaneous contraction. The biohybrid fish equipped with intrinsic control strategies demonstrated self-sustained body-caudal fin swimming, highlighting the role of feedback mechanisms in muscular pumps such as the heart and muscles.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Present address: Sue and Bill Gross Stem Cell Research Center, University of California, Irvine CA 92697, USA. These authors contributed equally to this work. Present address: Department of Chemical and Biomolecular Engineering, Henry Samueli School of Engineering, University of California Irvine, CA 92697, USA. Author contributions: K.Y.L. and S.-J.P. conceived and designed the study, developed a geometrically insulated cardiac tissue node-integrated muscular bilayer construct, designed and performed performance experiments, analyzed data, organized figures, and wrote the paper. K.K.P. conceived and designed the study, developed the idea of a geometrically insulated cardiac tissue node and muscular bilayer, and supervised the project. A.G.K. and G.V.L. contributed to the concept of a geometrically insulated cardiac tissue node and muscular bilayer, respectively. D.M. and G.V.L. contributed to the PIV experiments of both biohybrid and wild-type fish. S.L.K. designed optogenetic tools and edited the manuscript. C.M. assisted the fabrication of the biohybrid fish. S.L.K., J.Z., and H.A.M.A. performed primary neonatal rat ventricular harvest for the biohybrid fish optimization. All authors contributed to the preparation of the manuscript
ISSN:	0036-8075 1095-9203 1095-9203
DOI:	10.1126/science.abh0474