Sheep poxvirus identification by PCR in cell cultures

• Published in: Journal of virological methods Vol. 77; no. 1; pp. 75 - 79
• Main Authors: Mangana-Vougiouka, O., Markoulatos, P., Koptopoulos, G., Nomikou, K., Bakandritsos, N., Papadopoulos, O.
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 1999; Amsterdam Elsevier; New York, NY
• Subjects: Animals; Biological and medical sciences; Capripoxvirus; Cell Culture Techniques; Cell cultures; DNA, Viral - analysis; Fundamental and applied biological sciences. Psychology; Male; Microbiology; PCR; Polymerase Chain Reaction - methods; Poxviridae - genetics; Poxviridae - growth & development; Poxviridae - isolation & purification; Poxviridae Infections - veterinary; Poxvirus; Sensitivity and Specificity; Sheep; Sheep Diseases - virology; Sheep poxvirus; Sheep poxviruses; Techniques used in virology; Testis - cytology; Virology; Sheep poxviruses; PCR; Cell cultures; Performance evaluation; Chordopoxvirinae; Sheeppox virus; Method; Open reading frame; Infection; Virus; Polymerase chain reaction; Poxviridae; Capripoxvirus; Viral disease; Microorganism culture; Isolate; Ovine pox; Diagnosis; Veterinary; Genome; Inverted repeated sequence
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/S0166-0934(98)00138-4

A simple, rapid and specific diagnostic polymerase chain reaction (PCR) method was developed for sheep poxvirus identification. The primers used were from the sequenced genomes of the capripox viruses KS-1 and InS-1. Six different sheep pox isolates were tested against two orf (parapox) and three animal herpesviruses as controls. Material from uninfected cell cultures was also used as control.
The sensitivity of the PCR was approximately equivalent with each of the two primers and for the six sheep pox isolates. All the negative control virus DNAs were negative and differed clearly from those of the sheep pox strains.