Platelet-activating factor-induced NF-κB activation enhances VEGF expression through a decrease in p53 activity

• Published in: FEBS letters Vol. 580; no. 13; pp. 3006 - 3012
• Main Authors: Ko, Hyun-Mi, Jung, Hae Hyun, Seo, Kook Heon, Kang, Yeong-Rim, Kim, Han-A, Park, Sung Jun, Lee, Hern-Ku, Im, Suhn-Young
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 29-05-2006
• Subjects: NF-κB; nuclear factor kappaB; p53; p65 antisense oligonucleotide; p65 AS; p65 non-sense scrambled oligonucleotide; p65 NS; PAF; platelet-activating factor; vascular endothelial growth factor; VEGF; NF-κB; p65 AS; PAF; p65 NS; VEGF; p53
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2006.04.042

We investigated the role of p53 in nuclear factor (NF)-κB dependent, platelet-activating factor (PAF)-induced vascular endothelial growth factor (VEGF) expression. Transfected NF-κB subunits in ECV304 cells increased the tumor necrosis factor-α promoter activity, which was completely inhibited by p53. Transfected p53 increased p53RE promoter activity, which was completely inhibited by NF-κB subunits, indicating that cross-regulation occurs between NF-κB and p53. PAF-induced increase in VEGF expression was correlated with decreased p53 activity. These data suggest that NF-κB-dependency of the PAF-induced increase in VEGF expression is due to decreased p53 activity, which is reciprocally regulated by increased NF-κB activity.