Immunostimulatory effect of natural clinoptilolite as a possible mechanism of its antimetastatic ability

• Published in: Journal of cancer research and clinical oncology Vol. 128; no. 1; pp. 37 - 44
• Main Authors: PAVELIC, K, KATIC, M, SVERKO, V, MAROTTI, T, BOSNJAK, B, BALOG, T, STOJKOVIC, R, RADACIC, M, COLIC, M, POLJAK-BLAZI, M
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01-01-2002; Springer Nature B.V
• Subjects: Adjuvants, Immunologic; Animals; Antineoplastic agents; Antineoplastic Agents - therapeutic use; Biological and medical sciences; Cell Division - drug effects; Chemotherapy; Graft vs Host Reaction - immunology; Lymphocytes - immunology; Macrophages, Peritoneal - cytology; Macrophages, Peritoneal - drug effects; Macrophages, Peritoneal - physiology; Medical research; Medical sciences; Melanoma, Experimental - pathology; Mice; Mice, Inbred C57BL; Mice, Inbred CBA; Mice, Inbred Strains; N-Acetylneuraminic Acid - blood; Neoplasm Metastasis - prevention & control; NF-kappa B - metabolism; Oncology; Pharmacology. Drug treatments; Protein Subunits; Reference Values; Spleen - immunology; Superoxides - metabolism; Thiobarbituric Acid Reactive Substances - analysis; Zeolites - therapeutic use; Antineoplastic agent; Crystal form; Immunostimulation; Immune response; Treatment efficiency; Rodentia; Antimetastatic agent; Malignant tumor; Metastasis; Zeolite; Intragastric administration; Vertebrata; Chemotherapy; Mammalia; Treatment; Mouse; Animal; T-Lymphocyte; Malignant melanoma; Clinoptilolite; Micronization; Metastatic; Transcription factor NFκB; Mechanism of action
• ISSN: 0171-5216; 1432-1335
• DOI: 10.1007/s00432-001-0301-6

Many biochemical processes are closely related to ion exchange, adsorption, and catalysis. Zeolites reversibly bind small molecules such as oxygen or nitric oxide; they possess size and shape selectivity, the possibility of metalloenzyme mimicry, and immunomodulatory activity. These properties make them interesting for pharmaceutical industry and medicine.
The experiments were performed on mice. Different biochemical and molecular methods were used.
Micronized zeolite (MZ) administered by gastric intubation to mice injected with melanoma cells significantly reduced the number of melanoma metastases. In mice fed MZ for 28 days, concentration of lipid-bound sialic acid (LSA) in serum increased, but lipid peroxidation in liver decreased. The lymphocytes from lymph nodes of these mice provoked a significantly higher alogeneic graft-versus-host (GVH) reaction than cells of control mice. After i.p. application of MZ, the number of peritoneal macrophages, as well as their production of superoxide anion, increased. However, NO generation was totally abolished. At the same time, translocation of p65 (NFkappaB subunit) to the nucleus of splenic cells was observed.
Here we report antimetastatic and immunostimulatory effect of MZ and we propose a possible mechanism of its action.