Improved detection of microbial DNA after bead-beating before DNA isolation

Detection of microbial DNA in clinical samples by real-time PCR requires a universal extraction method with equally efficient lysis of cell walls of all possible microorganisms. We demonstrated that physical disruption by bead-beating with 0.1 mm beads in combination with MagNA Pure DNA III extracti...

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Bibliographic Details
Published in:	Journal of microbiological methods Vol. 80; no. 2; pp. 209 - 211
Main Authors:	de Boer, Richard, Peters, Remco, Gierveld, Sonja, Schuurman, Tim, Kooistra-Smid, Mirjam, Savelkoul, Paul
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 01-02-2010 Elsevier
Subjects:	Bacteriolysis Bead-beating Biological and medical sciences Cell walls Clinical Laboratory Techniques - methods DNA isolation DNA, Bacterial - isolation & purification Fundamental and applied biological sciences. Psychology Gram-Positive Bacteria - genetics Gram-Positive Bacteria - isolation & purification Gram-Positive Bacterial Infections - diagnosis MagNA Lyser MagNA Pure Microbiology Microspheres Molecular Diagnostic Techniques - methods Qiagen column Real-time PCR MagNA Lyser Bead-beating Qiagen column MagNA Pure Real-time PCR DNA isolation Polymerase chain reaction Isolation Detection Real time
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