Improved detection of microbial DNA after bead-beating before DNA isolation

• Published in: Journal of microbiological methods Vol. 80; no. 2; pp. 209 - 211
• Main Authors: de Boer, Richard, Peters, Remco, Gierveld, Sonja, Schuurman, Tim, Kooistra-Smid, Mirjam, Savelkoul, Paul
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01-02-2010; Elsevier
• Subjects: Bacteriolysis; Bead-beating; Biological and medical sciences; Cell walls; Clinical Laboratory Techniques - methods; DNA isolation; DNA, Bacterial - isolation & purification; Fundamental and applied biological sciences. Psychology; Gram-Positive Bacteria - genetics; Gram-Positive Bacteria - isolation & purification; Gram-Positive Bacterial Infections - diagnosis; MagNA Lyser; MagNA Pure; Microbiology; Microspheres; Molecular Diagnostic Techniques - methods; Qiagen column; Real-time PCR; MagNA Lyser; Bead-beating; Qiagen column; MagNA Pure; Real-time PCR; DNA isolation; Polymerase chain reaction; Isolation; Detection; Real time
• ISSN: 0167-7012; 1872-8359
• DOI: 10.1016/j.mimet.2009.11.009

Detection of microbial DNA in clinical samples by real-time PCR requires a universal extraction method with equally efficient lysis of cell walls of all possible microorganisms.
We demonstrated that physical disruption by bead-beating with 0.1
mm beads in combination with MagNA Pure DNA III extraction enhances microbial lysis of diverse Gram-positive microorganisms and may be used to optimize DNA extraction protocols in routine clinical diagnostics.