Chromosomal rearrangement features of Yersinia pestis strains from natural plague foci in China

The Yersinia pestis chromosome contains a large variety and number of insert sequences that have resulted in frequent chromosome rearrangement events. To identify the chromosomal rearrangement features of Y. pestis strains from five typical plague foci in China and study spontaneous DNA rearrangemen...

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Published in:	The American journal of tropical medicine and hygiene Vol. 91; no. 4; pp. 722 - 728
Main Authors:	Liang, Ying, Xie, Fang, Tang, Xinyuan, Wang, Mei, Zhang, Enmin, Zhang, Zhikai, Cai, Hong, Wang, Yanhua, Shen, Xiaona, Zhao, Hongqun, Yu, Dongzheng, Xia, Lianxu, Hai, Rong
Format:	Journal Article
Language:	English
Published:	United States The American Society of Tropical Medicine and Hygiene 01-10-2014
Subjects:	Animals Arvicolinae Base Sequence China Chromosomes, Bacterial - genetics Disease Reservoirs DNA Primers - genetics Gene Rearrangement Genome, Bacterial - genetics Genotype Geography Humans Murinae Phylogeny Plague - microbiology Polymerase Chain Reaction - methods Rats Sequence Analysis, DNA Yersinia pestis Yersinia pestis - classification Yersinia pestis - genetics Yersinia pestis - isolation & purification China
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Summary:	The Yersinia pestis chromosome contains a large variety and number of insert sequences that have resulted in frequent chromosome rearrangement events. To identify the chromosomal rearrangement features of Y. pestis strains from five typical plague foci in China and study spontaneous DNA rearrangements potentially stabilized in certain lineages of Y. pestis genomes, we examined the linking mode of locally collinear blocks (LCBs) in 30 Y. pestis strains by a polymerase chain reaction-based method. Our results suggest most strains have relatively stable chromosomal arrangement patterns, and these rearrangement characteristics also have a very close relationship with the geographical origin. In addition, some LCB linking modes are only present in specific strains. We conclude Y. pestis chromosome rearrangement patterns may reflect the genetic features of specific geographical areas and can be applied to distinguish Y. pestis isolates; furthermore, most of the rearrangement events are stable in certain lineages of Y. pestis genomes.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0002-9637 1476-1645
DOI:	10.4269/ajtmh.13-0491