Effect of Cyberlindnera jadinii supplementation on growth performance, serum immunity, antioxidant status, and intestinal health in winter fur-growing raccoon dogs ( Nyctereutes procyonoides )

• Published in: Frontiers in veterinary science Vol. 10; p. 1154808
• Main Authors: Zhao, Dehui, Zhang, Haihua, Liu, Keyuan, Wu, Yan, Zhang, Borui, Ma, Cuiliu, Liu, Hanlu
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 11-05-2023
• Subjects: antioxidant; Cyberlindnera jadinii; growth performance; immunity; microbiota; raccoon dogs; Veterinary Science; microbiota; growth performance; antioxidant; immunity; raccoon dogs; Cyberlindnera jadinii
• ISSN: 2297-1769; 2297-1769
• DOI: 10.3389/fvets.2023.1154808

This study aimed to investigate the effects of
supplementation on the growth performance, nutrient utilization, serum biochemistry, immunity, antioxidant status, and intestinal microbiota of raccoon dogs during the winter fur-growing period.
Forty-five 135 (±5) day-old male raccoon dogs were randomly assigned to three dietary groups supplemented with 0 (group N), 1 × 10
(group L) and 5 × 10
CFU/g (group H)
, with 15 raccoon dogs per group.
The results showed that
in groups L and H improved average daily gain (ADG) and decreased feed-to-weight ratio (F/G) (
< 0.05). No significant difference was found in nutrient digestibility and nitrogen metabolism among the three groups (
> 0.05). Compared with group N, serum glucose levels were lower in groups L and H (
< 0.05). The levels of serum immunoglobulins A and G in group L were higher than those in the other two groups (
< 0.05), and the levels of serum immunoglobulins A and M in group H were higher than those in group N (
< 0.05). Supplementation with
in groups L and H increased serum superoxide dismutase activity, and the total antioxidant capacity in group H increased compared with group N (
< 0.05). The phyla Bacteroidetes and Firmicutes were dominant in raccoon dogs. The results of principal coordinate analysis (PCoA) showed that the composition of microbiota in the three groups changed significantly (
< 0.05). The relative abundance of Campylobacterota was increased in the H group compared to the N and L groups (
< 0.05). The relative abundance of
was increased in group L compared with the other two groups (
< 0.05), while the relative abundance of
and
were decreased in group H compared with the other two groups (
< 0.05). Also, the relative abundance of
and
was higher in group L (
< 0.05) compared with group H.
In conclusion, dietary supplementation with
improved growth performance, antioxidant activity, immune status, and improved intestinal microbiota in winter fur-growing raccoon dogs. Among the concentrations tested, 1 × 10
CFU/g was the most effective level of supplementation.