Pituitary folliculo-stellate-like cell line produces a cytokine-induced neutrophil chemoattractant

• Published in: Neuropeptides (Edinburgh) Vol. 31; no. 1; pp. 46 - 51
• Main Authors: Zhang, Z.X, Koike, K, Sakamoto, Y, Jikihara, H, Kanda, Y, Inoue, K, Hirota, K, Miyake, A
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01-02-1997; Elsevier
• Subjects: Animals; Antibody Specificity; Biological and medical sciences; Blotting, Northern; Cell Line; Chemokines, CXC; Chemotactic Factors - genetics; Chemotactic Factors - immunology; Chemotactic Factors - metabolism; Culture Media, Conditioned - pharmacology; Cytoplasm - chemistry; Dose-Response Relationship, Drug; Enzyme-Linked Immunosorbent Assay; Fundamental and applied biological sciences. Psychology; Gene Expression - drug effects; Growth Inhibitors - analysis; Growth Inhibitors - metabolism; Growth Substances - genetics; Growth Substances - immunology; Growth Substances - metabolism; Hormones and neuropeptides. Regulation; Hypothalamus. Hypophysis. Epiphysis. Urophysis; Immunohistochemistry; Intercellular Signaling Peptides and Proteins; Lipopolysaccharides - pharmacology; Pituitary Gland - cytology; Pituitary Gland - immunology; Pituitary Gland - metabolism; Rats; RNA, Messenger - analysis; Tumor Necrosis Factor-alpha - pharmacology; Vertebrates: endocrinology; Adenohypophysis; Chemokine; Cell line; Neutrophil; Chemotactic factor; Cytokine
• ISSN: 0143-4179; 1532-2785
• DOI: 10.1016/S0143-4179(97)90019-6

In previous studies, we reported that cytokine-induced neutrophil chemoattractant (CINC) was produced in the pituitary gland. Here we investigated the possibility of detection of CINC immunoreactivity in the pituitary folliculostellate (FS)-like cell line (
TtT
GF
). Intense immunoreactivity was observed by immunocytochemistry in the cytoplasm and cell processes of
TtT
GF
cells. CINC immunoreactivity was detected by an enzyme-linked immunosorbent assay in as little as 3 h after conditioning the medium with
TtT
GF
cells, and it increased significantly in a time-dependent manner during the first 24 h of the culture. This immunoreactivity could be induced by lipopolysaccharide and tumor necrosis factor-α in a time- and dose-dependent manner. We also observed the expression of CINC mRNA in
TtT
GF
cells and LPS increased CINC mRNA accumulation in
TtT
GF
cells. These findings indicate that CINC produced by FS cells may play a role as a paracrine factor of the anterior pituitary gland, and
TtT
GF
will provide a useful model system for studying the regulation of CINC secretion by FS cells.