The in vivo expression patterns of individual type I interferon genes in murine cytomegalovirus infections

• Published in: Antiviral research Vol. 34; no. 1; pp. 17 - 26
• Main Authors: Yeow, Wen-Shuz, Lai, C.May, Beilharz, Manfred W
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01-03-1997; Elsevier
• Subjects: Animal viral diseases; Animals; Biological and medical sciences; Cytomegalovirus Infections - genetics; Genotype; In vivo; Infectious diseases; Interferon Type I - genetics; Liver - metabolism; Medical sciences; Mice; Mice, Inbred Strains; Murine cytomegalovirus; Muromegalovirus - genetics; Polymerase Chain Reaction; Reverse transcription-polymerase chain reaction; RNA, Messenger - genetics; Spleen - metabolism; Tissue-type; Type I murine interferon response; Viral diseases; Tissue-type; In vivo; Genotype; Type I murine interferon response; Reverse transcription-polymerase chain reaction; Spleen; Murine cytomegalovirus; Herpesviridae; Liver; Rodentia; Cytokine; Betaherpesvirinae; Virus; Infection; Vertebrata; Mammalia; Mouse; Animal; Interferon
• ISSN: 0166-3542; 1872-9096
• DOI: 10.1016/S0166-3542(96)01018-2

Type I interferon (IFN) family has more than 10 structurally related subtypes of alpha IFN (
IFNA) genes and a single beta IFN (
IFNB) gene. This study examined the expression of
MuIFNA1,
A4,
A5,
A6,
A9 and
MuIFNB mRNAs in the livers and spleens of MCMV-infected mice at 2, 4 and 6 h post infection. The three strains of inbred mice studied were C57BL/6, B6.C-
H-28
c
and BALB/c. B6.C-
H-28
c
strain is a congenic strain of C57BL/6 genomic background. Detection of the specific mRNAs was by an established semiquantitative procedure based on reverse transcription and PCR amplification followed by differential hybridization to specific oligonucleotides. Similar expression patterns of the type I IFN mRNAs were found in C57BL/6 and B6.C-
H-28
c
strains of mice. However, when the genotype was significantly different (BALB/c), a different expression pattern of IFN mRNAs was seen. Differences in the expression patterns of the type I IFN mRNAs was also seen between the livers and spleens of a given mouse strain. Thus, the present study indicates that mouse genotype appears to be a major determinant of the subtype response pattern seen in vivo and that tissue-type can influence the subtype response pattern seen within a given mouse genotype.