Fractionation of starch hydrolysates into dextrins with narrow molecular mass distribution and their detection by high-performance anion-exchange chromatography with pulsed amperometric detection

• Published in: Journal of Chromatography A Vol. 992; no. 1; pp. 75 - 83
• Main Authors: Gelders, Greta G, Bijnens, Lieve, Loosveld, Anne-Marie, Vidts, An, Delcour, Jan A
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 11-04-2003; Elsevier
• Subjects: Anion Exchange Resins; Applied sciences; Carbohydrates; Chromatography, High Pressure Liquid - methods; Chromatography, Ion Exchange - methods; Dextrins; Dextrins - chemistry; Dextrins - isolation & purification; Electrochemistry - methods; Exact sciences and technology; Glucooligosaccharides; Hydrolysis; Molecular Weight; Natural polymers; Organic polymers; Physicochemistry of polymers; Properties and characterization; Starch; Starch - chemistry; Starch and polysaccharides; Structure, morphology and analysis; Carbohydrates; Dialysis; Dextrins; Starch; Glucooligosaccharides; Molecular weight distribution; Oligosaccharide; Dextrin; Ion exchange chromatography; Electrochemical detector; Hydrolysate; Amperometry; Anion exchanger
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(03)00313-3

Low levels of high-molecular-mass dextrins in starch hydrolysates can be detected by high-performance anion-exchange chromatography with pulsed amperometric detection in spite of their low responses by dialysis of the starch hydrolysate and fractionation of the resulting adialysate with ethanol (final concentration 30–80% at 6
°C). In doing so, dextrin fractions with a relatively narrow molecular mass distribution were obtained.