Interleukin-10 gene promoter polymorphism in English and Polish healthy controls. Polymerase chain reaction haplotyping using 3' mismatches in forward and reverse primers

• Published in: Genes and immunity Vol. 1; no. 5; pp. 321 - 324
• Main Authors: Koss, K, Fanning, G C, Welsh, K I, Jewell, D P
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 01-06-2000
• Subjects: Alleles; Base Sequence; Cytokines; DNA Primers - genetics; England; Female; Gene Frequency; Gene polymorphism; Genes; Genotype & phenotype; Haplotypes; Histocompatibility antigen HLA; Humans; Interleukin 10; Interleukin-10 - genetics; Male; Poland; Polymerase Chain Reaction; Polymorphism; Polymorphism, Genetic; Primers; Promoter Regions, Genetic; United Kingdom; England; Poland; United Kingdom > UK
• ISSN: 1466-4879; 1476-5470
• DOI: 10.1038/sj.gene.6363669

A polymerase chain reaction with sequence-specific primers (PCR-SSP) system using primers with mismatches at the 3' ends was developed to determine polymorphisms in IL-10 promoter region. Three previously described biallelic polymorphisms in IL-10 were linked in a 12 reaction PCR-SSP system and the method used to provide genotype data on 233 UK and 166 Polish controls. There are eight possible polymorphic combinations in IL-10 promoter gene but only three were observed in both control groups. Population frequencies of IL-10 genotypes show, in contrast to HLA, that UK and Polish frequencies are remarkably similar.