Enantiomeric separation of acidic compounds of pharmaceutical interest by capillary electrochromatography employing glycopeptide antibiotic stationary phases

• Published in: Journal of Chromatography A Vol. 994; no. 1; pp. 227 - 232
• Main Authors: Fanali, Salvatore, Catarcini, Paolo, Presutti, Carla
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 25-04-2003; Elsevier
• Subjects: Acids; Analysis; Anti-Bacterial Agents - chemistry; Biological and medical sciences; Chromatography, Micellar Electrokinetic Capillary - methods; General pharmacology; Glycopeptides; Medical sciences; Nonsteroidal anti-inflammatory drugs; Pharmaceutical Preparations - chemistry; Pharmacology. Drug treatments; Stereoisomerism; Stationary phases, electrochromatography; Pharmaceutical analysis; Nonsteroidal anti-inflammatory drugs; Enantiomer separation; Nonsteroidal anti- inflammatory drugs; Separation; Chiral stationary phase; Vancomycin; Experimental study; Teicoplanin; Non steroidal antiinflammatory agent; Ultraviolet detector; Carboxylic acid; Optical resolution; Glycopeptide; Enantiomer; Electrochromatography; Arylpropionic acid derivatives
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(03)00439-4

Enantiomeric separation of some selected acidic compounds of pharmaceutical interest belonging to the group of non-steroidal anti-inflammatory drugs were separated by capillary electrochromatography employing silica based glycopeptide antibiotic stationary phases, namely vancomycin or a teicoplanin derivatives (Hepta-Tyr). The vancomycin stationary phase allowed to achieve the chiral resolution of some racemic studied compounds only using mobile phases containing ammonium formate at a relatively low pH 2.5–3.5 and acetonitrile. Employing the teicoplanin derivative stationary phase, good enantiomeric resolution was achieved eluting with mobile phases containing sodium phosphate pH 6–acetonitrile. Enantiomers were moved to the detector because a relatively high reversed electroosmotic flow (due to the positive charge of the stationary phase) and to the electrophoretic mobility of analytes.