Hibernating black bears ( Ursus americanus) experience skeletal muscle protein balance during winter anorexia

• Published in: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology Vol. 147; no. 1; pp. 20 - 28
• Main Authors: Lohuis, T.D., Harlow, H.J., Beck, T.D.I.
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01-05-2007
• Subjects: Animals; Anorexia - metabolism; Bears; DNA - analysis; Fasting; Female; Hibernation; Hibernation - physiology; Immobility; Male; Muscle Proteins - metabolism; Muscle, Skeletal - chemistry; Muscle, Skeletal - metabolism; Nitrogen - analysis; Nitrogen Isotopes; Protein Biosynthesis; Protein sparing; Protein synthesis and breakdown; Ribosomes - metabolism; RNA - analysis; Seasons; Ursidae - physiology; Immobility; Fasting; Protein synthesis and breakdown; Protein sparing; Hibernation; Bears
• ISSN: 1096-4959; 1879-1107
• DOI: 10.1016/j.cbpb.2006.12.020

Black bears spend four to seven months every winter confined to their den and anorexic. Despite potential for skeletal muscle atrophy and protein loss, bears appear to retain muscle integrity throughout winter dormancy. Other authors have suggested that bears are capable of net protein anabolism during this time. The present study was performed to test this hypothesis by directly measuring skeletal muscle protein metabolism during the summer, as well as early and late hibernation periods. Muscle biopsies were taken from the vastus lateralis of six free-ranging bears in the summer, and from six others early in hibernation and again in late winter. Protein synthesis and breakdown were measured on biopsies using
14C-phenylalanine as a tracer. Muscle protein, nitrogen, and nucleic acid content, as well as nitrogen stable isotope enrichment, were also measured. Protein synthesis was greater than breakdown in summer bears, suggesting that they accumulate muscle protein during periods of seasonal food availability. Protein synthesis and breakdown were both lower in winter compared to summer but were equal during both early and late denning, indicating that bears are in protein balance during hibernation. Protein and nitrogen content, nucleic acid, and stable isotope enrichment measurements of the biopsies support this conclusion.