Possible implication of macrophages in the regulation of cytochrome P450 activities in carp ( Cyprinus carpio)

• Published in: Fish & Shellfish Immunology Vol. 21; no. 1; pp. 80 - 91
• Main Authors: Marionnet, D., Deschaux, P., Reynaud, S.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-07-2006; Elsevier
• Subjects: Adjuvants, Immunologic - pharmacology; Animals; Biodiversity; Biodiversity and Ecology; Biotransformation activities; Carp; Carps - immunology; Carps - physiology; Cyprinus carpio; Cytochrome P-450 Enzyme System - biosynthesis; Cytochrome P-450 Enzyme System - drug effects; Cytochrome P-450 Enzyme System - metabolism; Dextran Sulfate - pharmacology; Ecology, environment; Environmental Sciences; Gene Expression Regulation - drug effects; Gene Expression Regulation - physiology; Immunostimulants; Kidney - chemistry; Kidney - drug effects; Leukocytes - drug effects; Life Sciences; Lipopolysaccharides - pharmacology; Liver - chemistry; Liver - drug effects; Macrophage-Activating Factors - biosynthesis; Macrophage-Activating Factors - drug effects; Macrophages; Macrophages - drug effects; Macrophages - immunology; Macrophages - physiology; MAF; Methylcholanthrene - administration & dosage; Methylcholanthrene - pharmacology; Modulation; Oxazines - analysis; Oxazines - metabolism; Respiratory Burst - drug effects; Respiratory Burst - physiology; ROS; Spleen - chemistry; Spleen - drug effects; Tilorone - pharmacology; G6P; EROD; Carp; DTT; Cyp 1A; 3-MC; Immunostimulants; DMBA; TCDD; GST; Biotransformation activities; Macrophages; BaP; MAF; Ah-R; PMSF; Modulation; HEPES; ROS; PAHs; NADP; alpha-NF; organs; Necrosis-factor-alpha; immune; hepatic cytochrome-p450; modulation; immunostimulants; drug biotransformation; biotransformation activities; macrophages; carp; rat-liver; dextran sulfate; mouse-liver; bacterial-endotoxin; down-regulation; oxidative stress
• ISSN: 1050-4648; 1095-9947; 1365-2567
• DOI: 10.1016/j.fsi.2005.10.006

Macrophages play a key role in the regulation of cytochrome P450 activity induced by immunostimulants in mammals. We investigated the effects of immunostimulants (LPS, dextran sulfate and tilorone) on biotransformation and macrophage activities in carp. The major effect of LPS was its capacity to inhibit 3-MC-induced cytochrome P450 activities in the liver and head kidney. Basal phase I activities were reduced by tilorone and dextran sulfate in immune organs. Tilorone and dextran sulfate differently modulated total cytochrome P450 contents and P4501A activities suggesting differential sensitivity for P450 classes. In immune organs, tilorone and dextran sulfate inhibited basal EROD activity. Tilorone inhibited 3-MC-induced EROD activity whereas dextran sulfate enhanced this activity. LPS and dextran sulfate increased ROS production by macrophages and all the immunostimulants induced macrophage activating factor (MAF) production. This study demonstrates for the first time in fish the capacity of CYP-regulated immunostimulants to activate macrophages and provides initial insight into the capacity of macrophages to regulate CYP activity induced by immunostimulants in fish.