Laparoscopic sleeve gastrectomy ameliorates mRNA expression of inflammation-related genes in subcutaneous adipose tissue but not in peripheral monocytes of obese patients

• Published in: Molecular and cellular endocrinology Vol. 383; no. 1-2; pp. 96 - 102
• Main Authors: Trachta, P., Dostálová, I., Haluzíková, D., Kasalický, M., Kaválková, P., Drápalová, J., Urbanová, M., Lacinová, Z., Mráz, M., Haluzík, M.
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 05-03-2014
• Subjects: Adipose tissue; Adipose tissues; Adult; Chemokine receptors; Chemokines, CC - genetics; Chemokines, CC - metabolism; Cytokines; Female; Gastrectomy - methods; Gene Expression; Gene Expression Profiling; Genes; Humans; Inflammation - genetics; Inflammation - metabolism; Inflammation - pathology; Inflammation - surgery; Interleukin-10 - genetics; Interleukin-10 - metabolism; Interleukin-18 - genetics; Interleukin-18 - metabolism; Laparoscopic sleeve gastrectomy; Laparoscopy; Middle Aged; Monocytes - metabolism; Monocytes - pathology; Obesity; Obesity - genetics; Obesity - metabolism; Obesity - pathology; Obesity - surgery; Organ Specificity; Patients; Peripheral monocytes; Receptors, CCR - genetics; Receptors, CCR - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sleeves; Subcutaneous Fat - metabolism; Subcutaneous Fat - pathology; Surgery; Toll-Like Receptor 2 - genetics; Toll-Like Receptor 2 - metabolism; Toll-Like Receptor 4 - genetics; Toll-Like Receptor 4 - metabolism; Weight Loss; LSG; Obesity; Adipose tissue; SCAT; Peripheral monocytes; PM; Laparoscopic sleeve gastrectomy
• ISSN: 0303-7207; 1872-8057
• DOI: 10.1016/j.mce.2013.11.013

•Subcutaneous fat of obese exerts a strong chemoattracting profile.•Peripheral monocytes complement this profile with corresponding receptors.•Laparoscopic sleeve gastrectomy reduced systemic low-grade inflammation.•LSG improved proinflammatory and chemotactic profile only in SCAT.
Low-grade inflammation links obesity, insulin resistance, and cardiovascular diseases. We investigated the effects of laparoscopic sleeve gastrectomy (LSG) on expression profile of genes involved in inflammatory pathways in subcutaneous adipose tissue (SCAT) and peripheral monocytes (PM). At baseline, obese group had significantly increased mRNA expression of proinflammatory chemokines (CCL-3, -17, -22), chemokine receptor CCR1 and cytokines (IL-10, IL-18) in SCAT and chemokine and other proinflammatory receptors (CCR-1, -2, -3, TLR-2, -4) in PM relative to control group. LSG decreased body weight, improved metabolic profile and reduced mRNA expression of up-regulated chemokine receptors, chemokines and cytokines in SCAT. In contrast, expression profiles in PM were largely unaffected by LSG. We conclude that LSG improved proinflammatory profile in subcutaneous fat but not in peripheral monocytes. The sustained proinflammatory and chemotactic profile in PM even 2years after LSG may contribute to partial persistence of metabolic complications in obese patients after metabolic surgery.