Acute-phase protein expression in DMSO-intoxicated rats

• Published in: Toxicology letters Vol. 147; no. 2; pp. 153 - 159
• Main Authors: Ivanović Matić, Svetlana, Dinić, Svetlana, Mihailović, Mirjana, Grigorov, Ilijana, Bogojević, Desanka, Poznanović, Goran
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01-03-2004; Amsterdam Elsevier Science
• Subjects: Acute-Phase Proteins - biosynthesis; Acute-Phase Proteins - immunology; Acute-phase reactants; acute-phase response; Animals; Biological and medical sciences; Corticosterone; Corticosterone - blood; Dimethyl Sulfoxide - toxicity; DMSO; General aspects. Methods; Immunoblotting; Immunoelectrophoresis, Two-Dimensional; Inflammation; Irritants - toxicity; Kinetics; Male; Medical sciences; Rats; Rats, Wistar; RNA, Messenger - analysis; RNA, Messenger - biosynthesis; Toxicology; Turpentine - toxicity; Inflammation; Acute-phase reactants; Corticosterone; DMSO; Rat; Steroid hormone; Acute; Rodentia; Protein; Organic solvent; Vertebrata; Methyl sulfoxide; Mammalia; Animal; Adrenal hormone
• ISSN: 0378-4274; 1879-3169
• DOI: 10.1016/j.toxlet.2003.11.011

The ability of dimethyl sulfoxide (DMSO) to induce the acute-phase (AP) response was examined. Injection of DMSO to laboratory rats caused a rapid doubling of the plasma corticosterone concentration 2
h after treatment. The elevated corticosterone concentration promoted the synthesis of mRNAs for several AP reactants. At 24
h after DMSO administration the relative serum concentration of cysteine-proteinase inhibitor (CPI) increased about 710%, α
1-acid glycoprotein (AGP) 630%, α
1-macroglobulin (MG) 510%, γ fibrinogen (Fb) 420%, haptoglobin (Hp) 280%, whereas the relative concentration of albumin, a “negative” AP reactant, decreased to 93%. The extent and kinetics of the corticosterone increase and the general increase of AP reactant mRNAs and protein serum concentrations after DMSO administration corresponded to the overall changes observed during the turpentine-induced AP response. On the basis of these findings it was concluded that DMSO was capable of promoting the AP response in rats.