Localisation of genes for resistance against Blumeria graminis f.sp. hordei and Puccinia graminis in a cross between a barley cultivar and a wild barley (Hordeum vulgare ssp. spontaneum) line

• Published in: Theoretical and applied genetics Vol. 106; no. 2; pp. 353 - 362
• Main Authors: BACKES, G, MADSEN, L. H, JAISER, H, STOUGAARD, J, HERZ, M, MOHLER, V, JAHOOR, A
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 2003; Berlin Springer Nature B.V
• Subjects: Ascomycota - physiology; Barley; Biological and medical sciences; Chromosome Mapping; Chromosomes, Plant; Classical genetics, quantitative genetics, hybrids; Fundamental and applied biological sciences. Psychology; Genes; Genes, Plant; Genetic Markers; Genetics of eukaryotes. Biological and molecular evolution; Health aspects; Hordeum - genetics; Plant Diseases - genetics; Pteridophyta, spermatophyta; Quantitative genetics; Quantitative Trait, Heritable; Recombination, Genetic; Vegetals; Germany; Barley; Blumeria graminis; Monocotyledones; Hordeum vulgare; Hordeum; Quantitative character; Puccinia hordei; QTL; Basidiomycetes; Erysiphe graminis; Cereal crop; Fungi; Quantitative trait loci; Resistance; Puccinia graminis; Gene; Gramineae; Resistance gene analogs; Analog; Angiospermae; Ascomycetes; Spermatophyta; Thallophyta; Cultivar
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-002-1148-1

The aims of this investigation have been to map new (quantitative) resistance genes against powdery mildew, caused by Blumeria graminis f.sp. hordei L., and leaf rust, caused by Puccinia hordei L., in a cross between the barley ( Hordeum vulgare ssp. vulgare) cultivar "Vada" and the wild barley ( Hordeum vulgare ssp. spontaneum) line "1B-87" originating from Israel. The population consisted of 121 recombinant inbred lines. Resistance against leaf rust and powdery mildew was tested on detached leaves. The leaf rust isolate "I-80" and the powdery mildew isolate "Va-4", respectively, were used for the infection in this experiment. Moreover, powdery mildew disease severity was observed in the field at two different epidemic stages. In addition to other DNA markers, the map included 13 RGA (resistance gene analog) loci. The structure of the data demanded a non-parametric QTL-analysis. For each of the four observations, two QTLs with very high significance were localised. QTLs for resistance against powdery mildew were detected on chromosome 1H, 2H, 3H, 4H and 7H. QTLs for resistance against leaf rust were localised on 2H and 6H. Only one QTL was common for two of the powdery mildew related traits. Three of the seven QTLs were localised at the positions of the RGA-loci. Three of the five powdery mildew related QTLs are sharing their chromosomal position with known qualitative resistance genes. All detected QTLs behaved additively. Possible sources of the distorted segregation observed, the differences between the results for the different powdery mildew related traits and the relation between qualitative and quantitative resistance are discussed.