Further characteristics of Arcanobacterium canis, a novel species of genus Arcanobacterium

• Published in: Veterinary microbiology Vol. 167; no. 3-4; pp. 619 - 622
• Main Authors: Sammra, O., Balbutskaya, A., Zhang, S., Hijazin, M., Nagib, S., Lämmler, C., Abdulmawjood, A., Prenger-Berninghoff, E., Kostrzewa, M., Timke, M.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 27-12-2013
• Subjects: Actinomycetales Infections - microbiology; Actinomycetales Infections - veterinary; Animals; Arcanobacterium; Arcanobacterium - classification; Arcanobacterium - genetics; Arcanobacterium canis; Canis; Cat; Cat Diseases - microbiology; Cats; desorption; DNA, Ribosomal Spacer - genetics; Dog; Dog Diseases - microbiology; Dogs; genes; Genotype; intergenic DNA; MALDI-TOF; mass spectrometry; Molecular identification; Molecular Sequence Data; otitis externa; Otitis Externa - microbiology; Otitis Externa - veterinary; Phylogeny; ribosomal DNA; RNA, Ribosomal, 16S - genetics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Arcanobacterium canis; MALDI-TOF; Dog; Cat; Molecular identification
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2013.09.017

Comparable to previously conducted phenotypical and genotypical investigations characterizing Arcanobacterium canis, a newly described species with the type strain A. canis DSM 25104 isolated from an otitis externa of a dog, four additional A. canis strains isolated from infections of three dogs and one cat could reliably be identified by phenotypic properties, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and by sequencing the genomic targets 16S rDNA, 16S-23S rDNA intergenic spacer region, 23S rDNA, and the genes rpoB and gap. All four A. canis investigated in the present study were isolated from the infected animals together with several other bacterial species indicating that the pathogenic importance of A. canis remains unclear. However, the detection of peptidic spectra by MALDI-TOF MS and the presented phenotypic and genotypic approaches might help to identify A. canis in future and might elucidate the role this species plays in infections of dogs and cats.