Mitogen-activated protein kinases contribute to temperature-induced cardiac remodelling in rainbow trout (Oncorhynchus mykiss)

• Published in: Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology Vol. 192; no. 1; pp. 61 - 76
• Main Authors: Ding, Y., Johnston, E. F., Gillis, T. E.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 2022; Springer Nature B.V
• Subjects: Acclimation; Acclimatization; Animal Physiology; Animals; Biochemistry; Biomedical and Life Sciences; Biomedicine; Cold; Cold acclimation; Collagen; Extracellular signal-regulated kinase; Female; Fibroblasts; Fish; Gelatinase A; Gene expression; Heart; Heart - physiology; Human Physiology; Hypertrophy; Kinases; Life Sciences; Male; Males; MAP kinase; Matrix metalloproteinase; Matrix metalloproteinases; Mitogen-Activated Protein Kinases - metabolism; Morphology; Oncorhynchus mykiss; Oncorhynchus mykiss - metabolism; Original Paper; Phosphorylation; Protein kinase; Proteins; Salmon; Signal transduction; Signaling; Temperature; Temperature dependence; Trout; Ventricular Remodeling; Water temperature; Zoology; p38 MAPK phosphorylation; Matrix metalloproteinases; Sex-specific effects; Thermal acclimation; Collagen deposition
• ISSN: 0174-1578; 1432-136X
• DOI: 10.1007/s00360-021-01406-5

Rainbow trout (
Oncorhynchus mykiss
) live in environments where water temperatures range between 4 °C and 20 °C. Laboratory studies demonstrate that cold and warm acclimations of male trout can have oppositional effects on cardiac hypertrophy and the collagen content of the heart. The cellular mechanisms behind temperature-induced cardiac remodelling are unclear, as is why this response differs between male and female fish. Studies with cultured trout cardiac fibroblasts suggests that collagen deposition is regulated, at least in part, by mitogen-activated protein kinase (MAPK) cell signalling pathways. We, therefore, hypothesized that temperature-dependent cardiac remodelling is regulated by these signalling pathways. To test this, male and female trout were acclimated to 18 °C (warm) in the summer and to 4 °C (cold) in the winter and the activation of MAPK pathways in the hearts were characterized and compared to that of control fish maintained at 12 °C. In addition, cardiac collagen content, cardiac morphology and the expression of gene transcripts for matrix metalloproteinases (MMP) -9, MMP-2, tissue inhibitor of matrix metalloproteinases and collagen 1α were characterized. p38 MAPK phosphorylation increased in the hearts of female fish with cold acclimation and the phosphorylation of extracellular signal-regulated kinase increased in the hearts of male fish with warm acclimation. However, there was no effect of thermal acclimation on cardiac morphology or collagen content in either male or female fish. These results indicate that thermal acclimation has transient and sex-specific effects on the phosphorylation of MAPKs but also how variable the response of the trout heart is to thermal acclimation.