High‐sensitivity chemiluminescent immunoassay investigation and application for the detection of T‐2 toxin and major metabolite HT‐2 toxin

• Published in: Journal of the science of food and agriculture Vol. 97; no. 3; pp. 818 - 822
• Main Authors: Li, Yanshen, Zhang, Jing, Mao, Xin, Wu, Yongtao, Liu, Gongzhen, Song, Liting, Li, Yonggang, Yang, Jianrong, You, Yanli, Cao, Xuelin
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01-02-2017; John Wiley and Sons, Limited
• Subjects: Antibodies, Monoclonal - metabolism; Carcinogens, Environmental - analysis; Carcinogens, Environmental - metabolism; Cereals; Chemiluminescence; China; CL‐ELISA; Contaminants; Cross Reactions; Enzyme-Linked Immunosorbent Assay; Food Contamination; Food contamination & poisoning; Food Inspection - methods; Food safety; Foods; high‐sensitivity; HT‐2 toxin; Immunoassay; Limit of Detection; Luminescent Measurements; metabolite; Metabolites; Methanol - chemistry; Mycotoxins; Oryza - chemistry; Reproducibility of Results; Seeds - chemistry; Solubility; Solvents - chemistry; T-2 Toxin - analogs & derivatives; T-2 Toxin - analysis; T-2 Toxin - metabolism; Toxins; T‐2 toxin; high-sensitivity; HT-2 toxin; T-2 toxin; metabolite; CL-ELISA
• ISSN: 0022-5142; 1097-0010
• DOI: 10.1002/jsfa.7801

BACKGROUND
T‐2 toxin is a widely distributed mycotoxin in cereals. HT‐2 toxin is the major metabolite, which is also a contaminant in cereals. T‐2 toxin and HT‐2 toxin have been identified as having carcinogenic, hepatotoxic, teratogenic and immunotoxic properties. To reduce the risk of contamination, a rapid, highly sensitive and inexpensive assay for the detection is required.
RESULTS
In this study a high‐sensitivity chemiluminescent enzyme‐linked immunoassay (CL‐ELISA) of T‐2 toxin and HT‐2 toxin was developed. With the help of the chemiluminescent substrate, this protocol showed a highly sensitive character with an IC50 as low as 33.28 ng mL−1 and 27.27 ng mL−1 for T‐2 and HT‐2, respectively. In addition, this method had no cross‐reaction with other structurally related mycotoxins.
CONCLUSION
These results indicated that the developed CL‐ELISA could be applied for the detection of T‐2 toxin and HT‐2 toxin in actual samples without complicated steps. © 2016 Society of Chemical Industry