Protective effects of curcumin on biochemical and molecular changes in sodium arsenite‐induced oxidative damage in embryonic fibroblast cells

• Published in: Journal of biochemical and molecular toxicology Vol. 33; no. 7; pp. e22320 - n/a
• Main Authors: Perker, Mehmet Can, Orta Yilmaz, Banu, Yildizbayrak, Nebahat, Aydin, Yasemin, Erkan, Melike
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01-07-2019
• Subjects: Antioxidants; Arsenite; Catalase; Cell viability; Curcumin; Cytotoxicity; Damage; Embryo fibroblasts; embryonic fibroblast; Enzymes; Fibroblasts; Gene expression; Genes; Glutathione; Glutathione peroxidase; Hydrogen peroxide; Hydroxyl radicals; Lipid peroxidation; Lipids; Oxidation; oxidative damage; Peroxidase; Peroxidation; Sodium; Sodium arsenite; Superoxide dismutase; Toxicity; embryonic fibroblast; sodium arsenite; oxidative damage; curcumin
• ISSN: 1095-6670; 1099-0461
• DOI: 10.1002/jbt.22320

The present study was aimed at determining the oxidative damage caused by sodium arsenite in 3T3 fibroblast cells and the possible protective role of curcumin (Cur) against sodium arsenite toxicity. Embryonic fibroblast cells were exposed to sodium arsenite (0.01, 0.1, 1, and 10 μM) in the presence and absence of Cur (2.5 μM) for 24 hours. Cell viability, cytotoxicity, lipid peroxidation, hydroxyl radical, hydrogen peroxide, antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase, and glutathione‐S‐transferase) and expression levels of antioxidant genes (superoxide dismutase, catalase, and glutathione peroxidase) were measured in embryonic fibroblast cells. Results demonstrated that sodium arsenite directly affects antioxidant enzymes and genes in 3T3 embryonic fibroblast cells and induces oxidative damage by increasing the amount of hydrogen peroxide, hydroxyl radical, and lipid peroxidation in the cell. Furthermore, the study indicated that Cur might be a potential ameliorative antioxidant to protect the fibroblast cell toxicity induced by sodium arsenite.