Transcriptome variations in human CaCo-2 cells: a model for enterocyte differentiation and its link to iron absorption

• Published in: Genomics (San Diego, Calif.) Vol. 83; no. 5; pp. 772 - 789
• Main Authors: Bédrine-Ferran, Hélène, Le Meur, Nolwenn, Gicquel, Isabelle, Le Cunff, Martine, Soriano, Nicolas, Guisle, Isabelle, Mottier, Stéphanie, Monnier, Annabelle, Teusan, Raluca, Fergelot, Patricia, Le Gall, Jean-Yves, Léger, Jean, Mosser, Jean
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01-05-2004; Elsevier
• Subjects: Biological and medical sciences; CaCo-2 cells; Cell Differentiation; Cell differentiation, maturation, development, hematopoiesis; Cell physiology; Classical genetics, quantitative genetics, hybrids; Enterocytes; Enterocytes - cytology; Enterocytes - metabolism; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling; Genes. Genome; Genetics of eukaryotes. Biological and molecular evolution; Genomics; Humans; Iron - metabolism; Iron overload; Methods, theories and miscellaneous; Microarray analysis of gene expression; Molecular and cellular biology; Molecular genetics; Oligonucleotide Array Sequence Analysis; Ontological annotations; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Messenger - metabolism; Transcription, Genetic - genetics; Iron overload; Ontological annotations; Enterocytes; Microarray analysis of gene expression; CaCo-2 cells; Cell differentiation; Genetic mapping; Human; Genomics; transcriptome; Iron; Models
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1016/j.ygeno.2003.11.014

Complete clinical expression of the HFE1 hemochromatosis is very likely modulated by genes linked to duodenal iron absorption, whose level is conditioned by unknown processes taking place during enterocyte differentiation. We carried out a transcriptomic study on CaCo-2 cells used as a model of enterocyte differentiation in vitro. Of the 720 genes on the microarrays, 80, 50, and 56 were significantly down-regulated up-regulated, and invariant during differentiation. With regard to iron metabolism, we showed that
HEPH,
SLC11A2,
SLC11A3, and
TF are significantly up-regulated, while
ATP7B and
SLC39A1 (and
SFT) are down-regulated and
ACO1,
dCYTb,
FECH, and
FTH1 show constant expression. Ontological annotations highlight the decrease in the expression of cell cycle and DNA metabolism associated genes as well as transcription, protein metabolism, signal transduction, and nucleocytoplasmic transport associated genes, whereas there are increases in the expression of genes linked to cell adhesion, lipid and xenobiotic metabolism, iron transport and homeostasis, and immune response.