In vivo anxiolytic and in vitro anti-inflammatory activities of water-soluble extract (WSE) of Nigella sativa (L.) seeds

• Published in: Natural product research Vol. 35; no. 16; pp. 2793 - 2798
• Main Authors: Babar, Z. M., Jaswir, I., Tareq, A. M., Ali Reza, A. S. M, Azizi, W. M., Hafidz, M., Ahfter, F., Hasan, M., Farhad, S., Uddin, M. M. Rokib, Ichwan, S.JA, Ahmed, Q. U., Taher, M., Mawa, I.
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis 12-08-2021; Taylor & Francis Ltd
• Subjects: anti-inflammatory; anxiolytic; Aspirin; Benzodiazepines; Biopolymer denaturation; Diazepam; Diclofenac; elevated plus maze (EPM); hole-board test (HBT); In vivo methods and tests; Inflammation; Lysis; Nigella sativa; Nigella sativa (L.) seeds; Protein denaturation; Proteins; RBC haemolysis; Seeds; WSE
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2019.1667348

The WSE is a highly polar, gummy and mucilaginous bioactive content of the Nigella sativa (L.) seeds. This study reports the anxiolytic and anti-inflammatory effects of WSE investigated using Elevated Plus Maze (EPM) and Hole-Board Test (HBT) in adult mice and human RBCs haemolysis inhibition and protein denaturation respectively. The oral WSE treatment (100 & 200 mg/kg b.w/day) for 72 hours has exhibited slightly better anxiolytic effect (p < 0.05) through the time span (92.33 & 93.33 s) spent in the opened arms of EPM vs. diazepam (1 mg/kg b.w i.p/day; 69.33 s). In HBT, only WSE (200 mg/kg b.w/day) has shown a promising number of mean head pokes (13.27 times/min) vs. diazepam (12.87 times/min). The WSE (62.5-500 µg/mL) exposure has exhibited 40.14-72.18% protection against lysis of RBCs vs. aspirin (57.04-71.48%) whilst 62.67-67.66% inhibition of protein denaturation vs. diclofenac sodium (43.11-80.64%). The current findings suggested WSE has promising anxiolytic and anti-inflammatory activities.