In vitro effects of Musa x paradisiaca extracts on four developmental stages of Haemonchus contortus

• Published in: Research in veterinary science Vol. 96; no. 1; pp. 127 - 132
• Main Authors: Marie-Magdeleine, C., Udino, L., Philibert, L., Bocage, B., Archimede, H.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-02-2014; Elsevier Limited; Elsevier
• Subjects: Adult worm motility; Animals; Anthelmintics - pharmacology; Banana tree; Chemical and Process Engineering; Chromatography, Thin Layer; Dose-Response Relationship, Drug; Egg hatching; Engineering Sciences; Food engineering; Gastro-intestinal nematodes; Guadeloupe; Haemonchus - growth & development; In vitro assay; In Vitro Techniques; L3 migration; Larva - growth & development; Larval development; Leaves; Life Sciences; Metabolites; Motility; Musa - chemistry; Phytotherapy - methods; Plant Extracts - pharmacology; Plant Leaves - chemistry; Plant Stems - chemistry; Regression Analysis; Veterinary medicine; Guadeloupe; Gastro-intestinal nematodes; Egg hatching; In vitro assay; L3 migration; Banana tree; Adult worm motility; Larval development
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2013.12.004

This study was carried out to evaluate the in vitro effect of Musa x paradisiaca stem and leaf against the parasitic nematode of small ruminants Haemonchus contortus. Three extracts (aqueous, methanolic and/or dichloromethane) of Musa x paradisiaca stem and leaf were tested in vitro on four developmental stages of H. contortus using egg hatch assay (EHA), larval development assay (LDA), L3 migration inhibition assay (LMI) and adult worm motility assay (AWM). The highly significant (P<0.0001) ability to stop larval development (inhibition >67% for each extract) and the negative effect of the dichloromethane extract of leaf on adult worm motility (43% of inhibition of motility after 24h of incubation) compared to the negative controls, suggest anthelmintic properties of Musa x paradisiaca stem and leaf against H. contortus. The active principles responsible for the activity could be secondary metabolites such as terpenoid and flavonoid compounds present in the leaf and stem of the plant.