Stable dicentric chromosomes induced by chemical mutagens in L5178Y mouse lymphoma cells

Stable, tandem dicentric chromosomes were discovered in two mutant cell colonies resulting from exposure of L5178Y mouse lymphoma cells to chemical mutagens. These unusual dicentrics were present in all metaphase cells examined from these colonies, even after approximately 65 cell generations in cul...

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Bibliographic Details
Published in:	Mutation research Vol. 173; no. 4; p. 263
Main Authors:	Blazak, W F, Stewart, B E, Galperin, I, Allen, K L, Rudd, C J, Mitchell, A D, Caspary, W J
Format:	Journal Article
Language:	English
Published:	Netherlands 01-04-1986
Subjects:	Acrolein - analogs & derivatives Acrolein - toxicity Anaphase - drug effects Animals Cells, Cultured Chromosome Aberrations - drug effects Hycanthone - analogs & derivatives Hycanthone - toxicity Karyotyping Leukemia L5178 - genetics Leukemia, Experimental - genetics Metaphase - drug effects Mice Mutagens Mutation
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Summary:	Stable, tandem dicentric chromosomes were discovered in two mutant cell colonies resulting from exposure of L5178Y mouse lymphoma cells to chemical mutagens. These unusual dicentrics were present in all metaphase cells examined from these colonies, even after approximately 65 cell generations in culture. Observation of cells in metaphase and anaphase suggests that the interstitial centromere in these dicentrics is non-functional, and that the terminal centromere is solely responsible for their orderly anaphase segregation.
ISSN:	0027-5107
DOI:	10.1016/0165-7992(86)90021-7