TP53 mutations in upper aerodigestive squamous cell carcinomas from a group of brazilian patients

• Published in: The American journal of surgery Vol. 170; no. 5; pp. 492 - 494
• Main Authors: Nagai, Maria A., Miracca, Elizabete C., Yamamoto, Lidia, Kowalski, Luiz P., Brentani, Ricardo R.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: New York, NY Elsevier Inc 01-11-1995; Elsevier; Elsevier Limited
• Subjects: Adult; Aged; Aged, 80 and over; Biological and medical sciences; Brazil; Cancer; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - pathology; Carcinoma, Squamous Cell - secondary; Conformation; Demographic variables; Deoxyribonucleic acid; DNA; DNA, Neoplasm - analysis; DNA, Neoplasm - genetics; Exons; Exons - genetics; Female; Gene Amplification; Genes, p53 - genetics; Genetics; Head and Neck Neoplasms - genetics; Head and Neck Neoplasms - pathology; Humans; Laryngeal Neoplasms - genetics; Laryngeal Neoplasms - pathology; Lymph nodes; Lymphatic Metastasis - genetics; Lymphatic Metastasis - pathology; Male; Medical sciences; Metastases; Middle Aged; Mouth Neoplasms - genetics; Mouth Neoplasms - pathology; Mutation; Neoplasm Staging; Otorhinolaryngology. Stomatology; p53 Protein; Pharyngeal Neoplasms - genetics; Pharyngeal Neoplasms - pathology; Point Mutation - genetics; Polymerase Chain Reaction; Polymorphism; Polymorphism, Single-Stranded Conformational; Respiratory system; Risk Factors; Smoking; Squamous cell carcinoma; Tobacco; Tobacco smoking; Tumor suppressor genes; Tumors; Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology; South America; Brazil; America; Squamous cell carcinoma; Upper aerodigestive tract; Mutation; Malignant tumor; DNA; Incidence
• ISSN: 0002-9610; 1879-1883
• DOI: 10.1016/S0002-9610(99)80337-7

Background: Loss of function of the tumor suppressor gene
TP53 contributes to the development of several tumors.
Patients and methods: We screened DNA samples from 47 patients with upper respiratory system squamous cell carcinomas for the presence of
TP53 mutations. Exons 4 to 8 of the
TP53 gene were amplified by the polymerase chain reaction, and mutations were identified by single-strand conformation polymorphism analysis.
Results: The
TP53 mutations were demonstrated in 23 cases (49%). Mutations were distributed as follows: exon 4, 5 cases; exon 5, 4 cases; exon 6, 6 cases; exon 7, 4 cases; and exon 8, 4 cases. Demographic variables, tumor site, stage, family history of cancer, and tobacco smoking were not predictors of
TP53 mutations. There was an increasing number of mutations in the more undifferentiated tumors (
P = 0.0595).
Conclusions: These findings suggest that
TP53 mutations are associated with tumor differentiation, but not with the risk of lymph node metastasis in the group of patients analyzed.