Continuous hydrolysis of raffinose family oligosaccharides in soymilk by fluidized bed reactor

• Published in: Food science & technology Vol. 43; no. 2; pp. 220 - 225
• Main Authors: Praveen Kumar, S.K., Mulimani, V.H.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01-03-2010; Elsevier
• Subjects: alpha-galactosidase; Aspergillus oryzae; Biological and medical sciences; bioreactors; continuous fermentation; Flatulence; fluidized beds; Food industries; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; fungal spores; hydrolysis; immobilized cells; oligosaccharides; raffinose; Raffinose family oligosaccharides; Soybean; soymilk; starter cultures; α-Galactosidase; Raffinose family oligosaccharides; Flatulence; Soybean; α-Galactosidase; Fluidized bed reactor; Enzyme; Continuous; Oligosaccharide; Soybean milk; Raffinose; Glycosylases; Hydrolysis; Grain legume; Glycosidases; Hydrolases
• ISSN: 0023-6438; 1096-1127
• DOI: 10.1016/j.lwt.2009.08.006

Soybean is an important pulse crop for human and animal nutrition. However, raffinose family oligosaccharides (RFOs) found in soybean seeds specifically hinder its consumption as it is not digested by normal human carbohydrases and is fermented by intestinal microflora, leading to flatulence. Immobilized spores of
Aspergillus oryzae were grown for 5 days in calcium alginate gel beads, then harvested and employed in RFOs hydrolysis in batch mode, repeated batch and continuous mode in a fluidized bed reactor. Effective hydrolysis of 244.4
mg/60
ml was obtained after 6
h incubation. And in continuous mode 465.6
mg/100
ml was obtained at a flow rate of 25
ml/h. The use of immobilized
A. oryzae cells for hydrolysis of RFOs is a promising solution to overcome flatulence and to increase the consumption of soy products.