Long-lived polyclonal B-cell lines derived from midgestation mouse embryo lymphohematopoietic progenitors reconstitute adult immunodeficient mice

• Published in: Blood Vol. 98; no. 6; pp. 1862 - 1871
• Main Authors: Martı́nez-M., José A., Minguet, Susana, Gonzalo, Pilar, Soro, Pilar G., de Andrés, Belén, Ízcue, Ana, Marcos, Miguel A.R., Gaspar, Marı́a-Luisa
• Format: Journal Article
• Language: English
• Published: Washington, DC Elsevier Inc 15-09-2001; The Americain Society of Hematology
• Subjects: 2,4-Dinitrophenol - immunology; Adoptive Transfer; Animals; B-Lymphocytes - transplantation; Biological and medical sciences; Cell Differentiation; Cell differentiation, maturation, development, hematopoiesis; Cell Line; Cell physiology; Clone Cells; Embryo, Mammalian - immunology; Fundamental and applied biological sciences. Psychology; Fundamental immunology; General aspects. Ontogeny. Phylogeny; Hematopoietic Stem Cells - immunology; Immunobiology; Immunoglobulin Fragments; Immunoglobulin Heavy Chains; Immunoglobulin M - biosynthesis; Interleukin-7 - pharmacology; Mice; Mice, Inbred BALB C; Mice, SCID; Molecular and cellular biology; Cell culture; Embryo; Lymphopoiesis; Stem cell; Rodentia; Hematopoietic cell; B-Lymphocyte; Cell differentiation; Long term; Interleukin 7; Vertebrata; Mammalia; Mouse; Hematopoiesis; Animal
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V98.6.1862

Lymphohematopoietic progenitors derived from midgestation mouse embryos were established in long-term cultures with stromal cell monolayers and interleukin 7 (IL-7), giving rise to B-lineage cell lines. The initial emergence and in vitro establishment of these early embryo cell lines were highly sensitive to IL-7–mediated signals, in comparison to cell lines similarly obtained using precursors from late fetal liver (> 13 days postcoitum) and adult bone marrow. The early embryo-derived progenitors spontaneously differentiated in vitro to CD19+IgM+ immature B cells in the presence of optimal concentrations of IL-7, in contrast to those progenitors obtained from late gestation and adult mice, whose differentiation only occurred in the absence of IL-7. The newly in vitro–generated B cells of the early embryo cell lines repopulated adult immunodeficient severe combined immunodeficient mice on their adoptive transfer in vivo and generated specific humoral immune responses after immunization.