Expression of protein IIIa of human adenovirus type 2 in Escherichia coli

We have constructed a plasmid encoding the protein IIIa gene of human adenovirus type 2. The gene was expressed under the control of the hybrid tac (trp-lac) promoter; the protein was synthesized at levels up to 5% of newly synthesized protein after IPTG induction. The protein IIIa produced in Esche...

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Bibliographic Details
Published in:	Gene Vol. 55; no. 2-3; p. 295
Main Authors:	Cuillel, M, Milleville, M, D'Halluin, J C
Format:	Journal Article
Language:	English
Published:	Netherlands 1987
Subjects:	Adenoviruses, Human - genetics Capsid Proteins Escherichia coli - genetics Phosphorylation Promoter Regions, Genetic Protein Processing, Post-Translational Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - genetics Recombinant Proteins - genetics Viral Proteins - biosynthesis Viral Proteins - genetics
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Summary:	We have constructed a plasmid encoding the protein IIIa gene of human adenovirus type 2. The gene was expressed under the control of the hybrid tac (trp-lac) promoter; the protein was synthesized at levels up to 5% of newly synthesized protein after IPTG induction. The protein IIIa produced in Escherichia coli has an apparent Mr on sodium dodecyl sulfate-polyacrylamide gels of 67 kDa, and was revealed with anti-adenovirus serum in Western blotting. The protein IIIa produced in bacteria was phosphorylated in the presence of [gamma-32P]ATP.
ISSN:	0378-1119
DOI:	10.1016/0378-1119(87)90289-7