Physiology, gene expression, and behavior as potential indicators of oxidative stress in piglets

• Published in: BMC veterinary research Vol. 20; no. 1; pp. 471 - 11
• Main Authors: Guevara, Raúl David, Pastor, Jose J, López-Vergé, Sergi, Manteca, Xavier, Tedo, Gemma, Llonch, Pol
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 16-10-2024; BioMed Central; BMC
• Subjects: Analysis; Animals; Antioxidant markers; Antioxidants; Behavior, Animal - drug effects; Catalase - genetics; Catalase - metabolism; Diseases; Female; Gene expression; Gene Expression - drug effects; Genes; Glutathione Peroxidase - genetics; Glutathione Peroxidase - metabolism; Glutathione transferase; Health aspects; Lipopolysaccharide; Lipopolysaccharides - pharmacology; Male; Oxidative stress; Oxidative Stress - drug effects; Physiological aspects; Pig behavior; Piglets; Rankings; Superoxide; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; Swine; Spain; Lipopolysaccharide; Oxidative stress; Piglets; Antioxidant markers; Gene expression; Pig behavior
• ISSN: 1746-6148; 1746-6148
• DOI: 10.1186/s12917-024-04320-4

The goal of the current study was to develop a pig model to investigate oxidative stress with a low negative impact on piglet welfare. Four independent trials (A, B, C, and D) were performed using a single intraperitoneal shot of lipopolysaccharide (LPS) as an immune challenge, aiming to assess the minimal LPS dose for piglets of different age to trigger a measurable acute oxidative stress response in healthy animals. In trial A, piglets received an LPS dose of 25 µg/KgBW at 41 days post-weaning (p.w.). In trial B, piglets received 25 µg/KgBW of LPS at 28 days p.w., in trials C And D, piglets were injected with 50 µg/KgBW of LPS at 21 days p.w., respectively. Piglets were randomly allocated either to the T1) Control group with saline solution (Ctrl), or T2) LPS challenge (LPS). The oxidative stress response was measured through the enzymatic activity of glutathione peroxidase (GPx), glutathione-S-transferase (GST), superoxide dismutase (SOD), and catalase (CAT), in both plasma and intestinal tissues. Intestinal gene expression of oxidative stress and inflammatory markers was assessed. Discomfort behaviors (panting, prostration, trembling, and vomits) were also recorded. Plasmatic and intestinal oxidative stress response was inconsistent across the four trials even when the dose and pig age were similar, possibly due to individual variability. Relative gene expression differences of anti-inflammatory cytokines (IL10), oxidation precursor (iNOS), and antioxidant markers (GPx4, MnSOD, and CAT) were detected between Ctrl and LPS treatment (P < 0.05) when assessed. Behavioral observations were sensitive to the LPS dose relative to Ctrl (P < 0.05) in all four trials. These results suggest that behavioral observations can be used as a non-invasive methodology to detect the presence of oxidative stress in pigs in challenging conditions. Behavioral observations were more sensitive than other indicators (i.e., biomarkers and gene expression) in the current study. However, a sensitivity scale system needs to be developed to qualify and rank the impact of oxidative stress in pigs.