Brown Alga Metabolites – Inhibitors of Marine Organism Fucoidan Hydrolases

• Published in: Chemistry of natural compounds Vol. 53; no. 2; pp. 345 - 350
• Main Authors: Silchenko, A. S., Imbs, T. I., Zvyagintseva, T. N., Fedoreyev, S. A., Ermakova, S. P.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-03-2017; Springer; Springer Nature B.V
• Subjects: Catabolism; Enzyme inhibitors; Enzymes; Glucosidase; Glycosidases; Hydrolases; Inhibitors; Medicine; Metabolites; Organic Chemistry; Pharmacy; Plant Sciences; Polysaccharides; inhibitors; enzymes; brown alga; fucoidan hydrolases; phlorotannins
• ISSN: 0009-3130; 1573-8388
• DOI: 10.1007/s10600-017-1985-4

Fucoidan hydrolase inhibitors were discovered for the first time. Metabolites in the aqueous EtOH extract of Fucus evanescens inhibited specifically the activity of enzymes involved in catabolism of brown-alga polysaccharides, i.e., fucoidanases (recombinant FFA from the marine bacterium Formosa algae KMM 3553T and PPF from the marine mollusk Patinopecten yessoensis) and glycosidases (recombinant α-L-fucosidase FucFa from F. algae and β-D-glucosidase G-II from the marine mollusk Littorina sitkana). The purified fucophlorethol fraction isolated from this extract inhibited recombinant fucoidanase FFA in the concentration range 10–50 μg/mL.