Molecular analysis of human immunoglobulin V lambda germline genes: subgroups V lambda III and V lambda IV

• Published in: Molecular immunology Vol. 29; no. 12; p. 1515
• Main Authors: Daley, M D, Peng, H Q, Misener, V, Liu, X Y, Chen, P P, Siminovitch, K A
• Format: Journal Article
• Language: English
• Published: England 01-12-1992
• Subjects: Amino Acid Sequence; Base Sequence; Cloning, Molecular; Gene Library; Genes, Immunoglobulin; Humans; Immunoglobulin lambda-Chains - genetics; Immunoglobulin Variable Region - genetics; Molecular Sequence Data; Pseudogenes; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; TATA Box
• ISSN: 0161-5890
• DOI: 10.1016/0161-5890(92)90226-N

Three human immunoglobulin V lambda germline genes have been isolated: two from the V lambda IV subgroup and one from the V lambda III subgroup. The V lambda III gene and one of the V lambda IV genes appear to be functional (each being utilized in at least two expressed V lambda genes), despite deviations from the reported consensus sequences in their promoter TATA-box and recombination signal sequence elements. The other V lambda IV gene is a pseudogene. Of the 20 human V lambda germline genes characterized to date, 45% are pseudogenes or vestigial genes.