Induction of adaptive response in human blood lymphocytes exposed to 900 MHz radiofrequency fields: Influence of cell cycle

• Published in: International journal of radiation biology Vol. 87; no. 9; pp. 993 - 999
• Main Authors: Sannino, Anna, Zeni, Olga, Sarti, Maurizio, Romeo, Stefania, Reddy, Siddharth B., Belisario, Maria Antonietta, Prihoda, Thomas J., Vijayalaxmi, Scarfi, Maria Rosaria
• Format: Journal Article
• Language: English
• Published: England Informa Healthcare 01-09-2011; Taylor & Francis
• Subjects: Adaptation, Physiological - drug effects; Adaptation, Physiological - genetics; Adaptation, Physiological - radiation effects; Adaptive response; Adult; cell cycle; Cell Cycle - drug effects; Cell Cycle - genetics; Cell Cycle - radiation effects; Cell Proliferation - drug effects; Cell Proliferation - radiation effects; Dose-Response Relationship, Drug; Dose-Response Relationship, Radiation; human blood lymphocytes; Humans; Lymphocytes - cytology; Lymphocytes - drug effects; Lymphocytes - metabolism; Lymphocytes - radiation effects; Male; micronuclei; Micronucleus Tests; Middle Aged; Mitomycin - pharmacology; Radio Waves - adverse effects; radiofrequency fields; Space life sciences; Time Factors; Young Adult
• ISSN: 0955-3002; 1362-3095
• DOI: 10.3109/09553002.2011.574779

Abstract
Purpose: To investigate the influence of cell cycle on the adaptive response (AR) induced by the exposure of human blood lymphocytes to radiofrequency fields (RF).
Materials and methods: Human peripheral blood lymphocytes in G0-, G1- or S-phase of the cell cycle were exposed for 20 hours to an adaptive dose (AD) of 900 MHz RF at an average specific absorption rate of 1.25 W/kg and then treated with a challenge dose (CD) of 100 ng/ml mitomycin C (MMC). Un-exposed and sham-exposed controls as well as cells treated with MMC alone were included in the study. The incidence of micronuclei (MN) was evaluated to determine the induction of AR.
Results: The results indicated that the cells which were exposed to AD of RF in G0- and G1-phase of the cell cycle did not exhibit AR while such a response was observed when the cells were exposed to AD of RF in S-phase of the cell cycle.
Conclusions: These results confirmed the observations reported in our previous investigation where AR was observed in human blood lymphocytes exposed to AD of RF in S-phase of the cell cycle and further suggested that the timing of AD exposure of RF is important to elicit AR.