Presence and properties of cAMP phosphodiesterase from promastigote forms of Leishmania tropica and Leishmania donovani

• Published in: Comparative biochemistry and physiology. B, Comparative biochemistry Vol. 93; no. 4; p. 789
• Main Authors: al-Chalabi, K A, Ziz, L A, al-Khayat, B
• Format: Journal Article
• Language: English
• Published: England 1989
• Subjects: 3',5'-Cyclic-AMP Phosphodiesterases - isolation & purification; 3',5'-Cyclic-AMP Phosphodiesterases - metabolism; Animals; Caffeine - pharmacology; Calcium - pharmacology; Hydrogen-Ion Concentration; Kinetics; Leishmania donovani - enzymology; Leishmania tropica - enzymology; Magnesium - pharmacology; Phenobarbital - pharmacology; Substrate Specificity; Temperature; Theophylline - pharmacology; Trifluoperazine - pharmacology
• ISSN: 0305-0491
• DOI: 10.1016/0305-0491(89)90046-1

1. This paper reports the isolation and the partial purification of cAMP phosphodiesterase (EC 3.1.4.17) from the promastigote form of Leishmania tropica and a preliminary result from Leishmania donovani. 2. The activity of the fraction obtained from column chromatography was measured. 3. The effects of pH, temperature, time of incubation and various compounds on its activity in vitro were obtained. 4. Two peaks (I and II) exhibiting cyclic nucleotide phosphodiesterase activity were obtained. 5. Both activities were found to require the addition of Mg2+ ions for full effect. 6. The apparent Km values for the first and second peaks were 1.43 x 10(-3) M and 4.1 x 10(-3) M respectively. L. donovani shows only one peak of activity.