Analysis of the stabilizing effect of epsilon-aminocaproic acid by electrophoretic techniques and immunoblotting

The effect of epsilon-aminocaproic acid (EACA) on the degradation of aqueous pollen extracts was studied by isoelectric focusing, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and immunoblotting. The extracts were stored at 4 degrees C for 7 days and at 37 degrees C for 1, 4, and 7 days...

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Bibliographic Details
Published in:	Journal of allergy and clinical immunology Vol. 83; no. 3; p. 601
Main Authors:	Van Hoeyveld, E M, Lejoly, M, Walravens, M J, Stevens, E A
Format:	Journal Article
Language:	English
Published:	United States 01-03-1989
Subjects:	Aminocaproates - pharmacology Aminocaproic Acid - pharmacology Drug Stability Electrophoresis, Polyacrylamide Gel - methods Humans Immunoblotting Isoelectric Focusing Plant Extracts - immunology Pollen Sodium Dodecyl Sulfate
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Summary:	The effect of epsilon-aminocaproic acid (EACA) on the degradation of aqueous pollen extracts was studied by isoelectric focusing, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and immunoblotting. The extracts were stored at 4 degrees C for 7 days and at 37 degrees C for 1, 4, and 7 days. Addition of 0.1 mol/L of EACA before storage partly protected the extract from degradation. The protective effect of EACA could be demonstrated most clearly by immunoblotting, suggesting that more epitopes were preserved in an antigenic configuration. The stabilizing effect increased with higher EACA concentrations.
ISSN:	0091-6749
DOI:	10.1016/0091-6749(89)90072-9