Digital image analysis using video microscopy of human-derived prostate cancer vs normal prostate organoids to assess migratory behavior on extracellular matrix proteins

• Published in: Frontiers in oncology Vol. 12; p. 1083150
• Main Authors: Marr, Kendra D, Ignatenko, Natalia A, Warfel, Noel A, Batai, Ken, Cress, Anne E, Pollock, Grant R, Wong, Ava C, Lee, Benjamin R
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 13-01-2023
• Subjects: cancer phenotypes; extracellular matrix; Oncology; organoids; prostate cancer; video microscopy; prostate cancer; video microscopy; cancer phenotypes; organoids; extracellular matrix
• ISSN: 2234-943X; 2234-943X
• DOI: 10.3389/fonc.2022.1083150

The advent of perpetuating living organoids derived from patient tissue is a promising avenue for cancer research but is limited by difficulties with precise characterization. In this brief communication, we demonstrate
time-lapse imaging distinct phenotypes of prostate organoids derived from patient material- without confirmation of cellular identity. We show that organoids derived from histologically normal tissue more readily spread on a physiologic extracellular matrix (ECM) than on pathologic ECM (p<0.0001), while tumor-derived organoids spread equally on either substrate (p=0.2406). This study is an important proof-of-concept to defer precise characterization of organoids and still glean information into disease pathology.