Natural killer activity of Kurloff cells: a direct demonstration on purified Kurloff cell suspensions

Natural killer activity of Kurloff cells: a direct demonstration on purified Kurloff cell suspensions

In order to study their natural killer effect, guinea pig splenic Kurloff cells were fractionated by Percoll discontinuous density gradient centrifugation. Kurloff cells were collected and tested for cytotoxicity in a 24-hr chromium-release test. Comparison of different splenic cellular samples (of...

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Bibliographic Details
Published in:Cellular immunology Vol. 87; no. 2; p. 674
Main Authors: Debout, C, Quillec, M, Izard, J
Format: Journal Article
Language:English
Published: Netherlands 01-01-1984
Subjects:
Animals
Cell Separation
Female
Guinea Pigs
Immunity, Innate
Killer Cells, Natural - cytology
Killer Cells, Natural - immunology
Male
Spleen - cytology
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Summary:In order to study their natural killer effect, guinea pig splenic Kurloff cells were fractionated by Percoll discontinuous density gradient centrifugation. Kurloff cells were collected and tested for cytotoxicity in a 24-hr chromium-release test. Comparison of different splenic cellular samples (of males or estrogenized females) with increasing percentage of Kurloff cells, revealed a highly significant positive correlation (r = 0.93, alpha less than 0.01) with the cellular cytotoxicity developed against the K 562 target cells.
ISSN:0008-8749
DOI:10.1016/0008-8749(84)90034-0