Quercetin, Siamois 1 and siamois 2 induce apoptosis in human breast cancer MDA-MB-435 cells xenograft in vivo

• Published in: Cancer biology & therapy Vol. 6; no. 1; pp. 56 - 61
• Main Authors: Dechsupa, Samarn, Kothan, Suchart, Vergote, Jackie, Leger, Gerard, Martineau, Antoine, Beranger, Simone, Kosanlavit, Rachian, Moretti, Jean-Luc, Mankhetkorn, Samlee
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 01-01-2007
• Subjects: Animals; Apoptosis; Binding; Biology; Bioscience; Breast Neoplasms - pathology; Calcium; Cancer; Cell; Cell Proliferation - drug effects; Cycle; Humans; Inhibitory Concentration 50; Landes; Mice; Mice, Nude; Organogenesis; Proteins; Quercetin - analogs & derivatives; Quercetin - pharmacology; Xenograft Model Antitumor Assays
• ISSN: 1538-4047; 1555-8576
• DOI: 10.4161/cbt.6.1.3548

We sought to investigate the apoptosis-inducing activities of quercetin, Siamois 1, and Siamois 2 against invasive estrogen-receptor negative MDA-MB 435 cells xenografted in athymic nude mice. This study clearly demonstrated that these compounds exhibited apoptosis-inducing activities in cell culture system. Quercetin (20 μg/mL), Siamois 1 (100 μg/mL), and Siamois 2 (200 μg/mL) can induce apoptotic cell death by 40± 5%, 44 ± 14 %, and 31 ± 13 %, respectively. Two-fold of IC50 of these compounds were clearly found to induce apoptosis in breast tumor tissue which can be determined by
99m
Tc-annexin V scintigraphy and histological staining. This is the first report that the apoptosis-inducing effects of quercetin, Siamois 1, and Siamois 2 on the MDA-MB 435 cell in vitro were effectively extrapolated to the in vivo situation. These compounds might be considered as a simple dietary supplement and with further clinical investigation for their use as a nutrition-based intervention in breast cancer treatment.