Development of SCAR and CAPS markers linked to a recessive male sterility gene in lettuce (Lactuca sativa L.)

• Published in: Euphytica Vol. 180; no. 3; pp. 429 - 436
• Main Authors: Hayashi, M., Ujiie, A., Serizawa, H., Sassa, H., Kakui, H., Oda, T., Koba, T.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-08-2011; Springer; Springer Nature B.V
• Subjects: Agronomy. Soil science and plant productions; Analysis; Biological and medical sciences; Biomarkers; Biomedical and Life Sciences; Biotechnology; Cloning; Fundamental and applied biological sciences. Psychology; Genes; Genetic aspects; Genetics and breeding of economic plants; Heterosis. Floral biology applications: apomixy, male sterility, incompatibility, varia; Hybrids; Lactuca sativa; Lettuce; Life Sciences; Male sterility; Plant breeding: fundamental aspects and methodology; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; CAPS marker; Lettuce; SCAR marker; Genetic male sterility; AFLP; Sequence characterized amplified region marker; Cleaved amplified polymorphic sequence; Compositae; Vegetable crop; Gene; Dicotyledones; Angiospermae; Genetic improvement; Genic male sterility; Male sterility; Spermatophyta; Lactuca sativa; Amplified fragment length polymorphism marker
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1007/s10681-011-0417-y

Genetic male sterility (GMS) has been a useful system for the production of hybrid varieties in self-pollinated plants. We obtained a GMS line developed from a spontaneous mutation in lettuce (
Lactuca sativa
L.). Genetic analysis in our previous study revealed that the sterility was controlled by a recessive gene which was named
ms
-
S
. For simple and quick screening of individuals showing male sterility, we attempted molecular mapping of the
ms
-
S
locus using an amplified fragment length polymorphism (AFLP) technique. From the examination of 4,096 AFLP primer combinations, 63 AFLP markers were found to be linked to the gene and nine of them were successfully converted into sequence characterized amplified region (SCAR) markers and cleaved amplified polymorphic sequence (CAPS) markers. Linkage analysis indicated that these nine markers were closely linked to the
ms
-
S
gene and all were located on the same side of the gene. The minimum genetic distance between the
ms
-
S
gene and a marker was 3.1 cM. These results provide additional information for map-based cloning of the
ms
-
S
gene and will be of great help for lettuce breeding using GMS to produce F
1
hybrids.