Favorable effect of hemodialysis on decreased serum antioxidant activity in hemodialysis patients demonstrated by electron spin resonance

• Published in: Journal of the American Society of Nephrology Vol. 8; no. 7; pp. 1157 - 1163
• Main Authors: NAGASE, S, AOYAGI, K, HIRAYAMA, A, GOTOH, M, UEDA, A, TOMIDA, C, KAMEZAKI, T, NAGAI, Y, KIKUCHI, H, KOYAMA, A
• Format: Journal Article
• Language: English
• Published: Hagerstown, MD Lippincott Williams & Wilkins 01-07-1997
• Subjects: Adult; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Antioxidants - metabolism; Biological and medical sciences; Case-Control Studies; Catalase - blood; Electron Spin Resonance Spectroscopy; Emergency and intensive care: renal failure. Dialysis management; Female; Humans; Hydroxyl Radical - blood; Intensive care medicine; Male; Medical sciences; Middle Aged; Reactive Oxygen Species - metabolism; Renal Dialysis; Uremia - blood; Uremia - therapy; Human; Extrarenal dialysis; Hemodialysis; Spin wave resonance; Biological effect; Case control study; Antioxidant; Experimental study; Hydroxyl; Peroxidation
• ISSN: 1046-6673; 1533-3450
• DOI: 10.1681/asn.v871157

There is considerable evidence that uremic patients are in a highly peroxidative state. The purpose of this study was to investigate the serum antioxidant activity that may regulate, or represent, the redox state in vivo. Serum from pre- and posthemodialysis patients and from healthy control subjects was added to a system generating the hydroxyl radical, and then the signal intensities of reactive oxygen species were measured by electron spin resonance and spin-trapping technique. The electron spin resonance signals of the reaction mixture containing prehemodialysis sera were significantly stronger than those of the reaction mixture containing healthy sera (P < 0.001, n = 19), and there was no significant difference in the signals between the reaction mixture containing posthemodialysis and healthy sera. These findings demonstrated that serum antioxidant activity in hemodialysis patients is significantly decreased, and this pathological condition is improved by hemodialysis treatment.