The Catalytic Mechanism of Tyrosine Phenol-Lyase from Erwinia herbicola: The Effect of Substrate Structure on pH-Dependence of Kinetic Parameters in the Reactions with Ring-Substituted Tyrosines

• Published in: Zeitschrift für Naturforschung C. A journal of biosciences Vol. 51; no. 5; pp. 363 - 370
• Main Authors: Faleev, N. G., Spirina, S.N., Ivoilov, V. S., Demidkina, T. V., Phillips, R. S.
• Format: Journal Article
• Language: English
• Published: Germany Verlag der Zeitschrift für Naturforschung 01-05-1996
• Subjects: Ammonium Sulfate; Chromatography, Gel; Citrobacter - enzymology; Electrophoresis, Polyacrylamide Gel; Erwinia - enzymology; Erwinia herbicola; Hydrogen-Ion Concentration; Kinetics; Mathematics; Mechanism; Models, Theoretical; pH -Dependence; Protamines; Substrate Specificity; Substrate Structure; Tyrosine - analogs & derivatives; Tyrosine - metabolism; Tyrosine Phenol-Lyase; Tyrosine Phenol-Lyase - isolation & purification; Tyrosine Phenol-Lyase - metabolism
• ISSN: 0939-5075; 1865-7125
• DOI: 10.1515/znc-1996-5-613

Apparently homogeneous tyrosine phenol-lyase (TPL) from Erwinia herbicola has been prepared by a new method. The pH-dependencies of the main kinetic parameters for the reactions of Erwinia TPL with tyrosine, 2-fluorotyrosine, 3-fluorotyrosine, 2-chlorotyrosine, and 3.4-dihydroxyphenylalanine (DOPA ) have been studied. The pattern of pH-dependence of V
depends on the nature of the substituent in the aromatic ring. For the substrates bearing small substituents (H, 2-F, 3-F) K
x values were found to be pH-independent. For 2-chlorotyrosine and DOPA V
decreased at lower pH, the effect being described by equation with one pKa. Generally two bases are reflected in the pH dependence of V
/K
. The first base, probably is responsible for the abstraction of a-proton, while the second one, interacts with the phenolic hydroxyl at the stage of binding. The reaction of TPL with DOPA differs from the reactions with other tyrosines by the requirement of an additional base which is reflected in the pH-profiles of both and V
/K
. For the reaction of TPL from Citrobacter intermedins with DOPA only V
/K
values could be determined. The activity of Citrobacter enzyme towards DOPA is considerably less than that of E. herbicola enzyme, and its maximal value is attained at higher pH.